دورية أكاديمية
Frequency of azole resistance in clinical and environmental strains of Aspergillus fumigatus in Turkey: A multicentre study
| العنوان: | Frequency of azole resistance in clinical and environmental strains of Aspergillus fumigatus in Turkey: A multicentre study |
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| المؤلفون: | Ener B., Ergin Ç., Gülmez D., A?ca H., TikveÅŸli M., Aksoy S.A., Otkun M., Sig, Ali Korhan, Ogunc, Dilara, Ozhak, Betil, Topac, Tuncay, Ozdemir, Asli, Metin, Dilek Yesim, Polat, Suleyha Hilmioglu, Oz, Yasemin, Koc, Nedret, Atalay, Mustafa Altay, Erturan, Zayre, Birinci, Asuman, Cerikcioglu, Nilgun, Timur, Demet, Eksi, Fahriye, Genc, Gonca Erkose, Findik, Duygu, Gurcan, Saban, Kalkanci, Ayse, Arikan-Akdagli, Sevtap |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2022 |
| المجموعة: | Pamukkale University Repository / Pamukkale Üniversitesi Açık Erişim Arşivi |
| مصطلحات موضوعية: | itraconazole, posaconazole, pyrrole, voriconazole, antifungal agent, fungal protein, pyrrole derivative, antifungal resistance, Article, Aspergillus fumigatus, controlled study, fungal gene, fungal strain, fungus growth, fungus isolation, fungus mutation, gene mutation, genotyping, microsatellite marker, minimum inhibitory concentration, molecular phylogeny, nonhuman, Turkey (republic), prevalence, prospective study, screening, aspergillosis, clinical trial, genetics, human |
| الوصف: | Objectives: Aspergillus fumigatus causes several diseases in humans and azole resistance in A. fumigatus strains is an important issue. The aim of this multicentre epidemiological study was to investigate the prevalence of azole resistance in clinical and environmental A. fumigatus isolates in Turkey. Methods: Twenty-one centres participated in this study from 1 May 2018 to 1 October 2019. One participant from each centre was asked to collect environmental and clinical A. fumigatus isolates. Azole resistance was screened for using EUCAST agar screening methodology (EUCAST E.DEF 10.1) and was confirmed by the EUCAST E.DEF 9.3 reference microdilution method. Isolates with a phenotypic resistance pattern were sequenced for the cyp51A gene and microsatellite genotyping was used to determine the genetic relationships between the resistant strains. Results: In total, resistance was found in 1.3% of the strains that were isolated from environmental samples and 3.3% of the strains that were isolated from clinical samples. Mutations in the cyp51A gene were detected in 9 (47.4%) of the 19 azole-resistant isolates, all of which were found to be TR34/L98H mutations. Microsatellite genotyping clearly differentiated the strains with the TR34/L98H mutation in the cyp51A gene from the strains with no mutation in this gene. Conclusions: The rate of observed azole resistance of A. fumigatus isolates was low in this study, but the fact that more than half of the examined strains had the wild-Type cyp51A gene supports the idea that other mechanisms of resistance are gradually increasing. © 2022 The Author(s) 2022. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0305-7453 |
| العلاقة: | Journal of Antimicrobial Chemotherapy; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://doi.org/10.1093/jac/dkac125Test; https://hdl.handle.net/11499/47554Test; 77; 1894; 1898; 2-s2.0-85133214337; WOS:000784578100001 |
| DOI: | 10.1093/jac/dkac125 |
| الإتاحة: | https://doi.org/10.1093/jac/dkac125Test https://hdl.handle.net/11499/47554Test |
| حقوق: | none |
| رقم الانضمام: | edsbas.C28C2CEE |
| قاعدة البيانات: | BASE |
| تدمد: | 03057453 |
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| DOI: | 10.1093/jac/dkac125 |