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A comparative study on eDNA-based detection of Siamese bat catfish (Oreoglanis siamensis) in wet and dry conditions

التفاصيل البيبلوغرافية
العنوان: A comparative study on eDNA-based detection of Siamese bat catfish (Oreoglanis siamensis) in wet and dry conditions
المؤلفون: Maslin Osathanunkul, Chatmongkon Suwannapoom
المصدر: Scientific Reports, Vol 14, Iss 1, Pp 1-8 (2024)
بيانات النشر: Nature Portfolio, 2024.
سنة النشر: 2024
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: eDNA analysis, Endemic species, Freshwater species detection, Rainfall influence, Siamese bat catfish, Medicine, Science
الوصف: Abstract The use of environmental DNA (eDNA) analysis has demonstrated notable efficacy in detecting the existence of freshwater species, including those that are endangered or uncommon. This application holds significant potential for enhancing environmental monitoring and management efforts. However, the efficacy of eDNA-based detection relies on several factors. In this study, we assessed the impact of rainfall on the detection of eDNA for the Siamese bat catfish (Oreoglanis siamensis). Quantitative polymerase chain reaction (qPCR) analysis indicated that samples from days with average rainfall exceeding 35 mm (classified as heavy and very heavy rain) yielded negative results. While eDNA detection remains feasible on light or moderate rainy days, a noteworthy reduction in eDNA concentration and qPCR-positive likelihood was observed. Analysis across 12 sampling sites established a statistically significant negative relationship (p
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2045-2322
العلاقة: https://doaj.org/toc/2045-2322Test
DOI: 10.1038/s41598-024-58752-x
الوصول الحر: https://doaj.org/article/e2b115434b614ad1a282539e8ee6cb4aTest
رقم الانضمام: edsdoj.2b115434b614ad1a282539e8ee6cb4a
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20452322
DOI:10.1038/s41598-024-58752-x