التفاصيل البيبلوغرافية
| العنوان: |
Tear-Based Vibrational Spectroscopy Applied to Amyotrophic Lateral Sclerosis |
| المؤلفون: |
Diletta Ami (312344), Alessandro Duse (11836984), Paolo Mereghetti (19517), Federica Cozza (7820198), Francesca Ambrosio (6052745), Erika Ponzini (11836987), Rita Grandori (249174), Christian Lunetta (202352), Silvia Tavazzi (1844740), Fabio Pezzoli (1686127), Antonino Natalello (111135) |
| سنة النشر: |
1753 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Medicine, Genetics, Cancer, Space Science, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Physical Sciences not elsewhere classified, Information Systems not elsewhere classified, sensitivity (∼ 88, fourier transform infrared, amyotrophic lateral sclerosis, optical spectroscopy techniques, effective biomarkers combined, tears allowed us, human tears opens, vibrational spectroscopy applied, vibrational approach made, vibrational spectroscopy, raman spectroscopy, proposed approach, als biomarkers, ∼ 100, unaccomplished identification, revolutionize diagnostics, raman microspectroscopies, precision medicine, phenylalanine level, new route, neurodegenerative disorders, neurodegenerative diseases |
| الوصف: |
Biofluid analysis by optical spectroscopy techniques is attracting considerable interest due to its potential to revolutionize diagnostics and precision medicine, particularly for neurodegenerative diseases. However, the lack of effective biomarkers combined with the unaccomplished identification of convenient biofluids has drastically hampered optical advancements in clinical diagnosis and monitoring of neurodegenerative disorders. Here, we show that vibrational spectroscopy applied to human tears opens a new route, offering a non-invasive, label-free identification of a devastating disease such as amyotrophic lateral sclerosis (ALS). Our proposed approach has been validated using two widespread techniques, namely, Fourier transform infrared (FTIR) and Raman microspectroscopies. In conjunction with multivariate analysis, this vibrational approach made it possible to discriminate between tears from ALS patients and healthy controls (HCs) with high specificity (∼97% and ∼100% for FTIR and Raman spectroscopy, respectively) and sensitivity (∼88% and ∼100% for FTIR and Raman spectroscopy, respectively). Additionally, the investigation of tears allowed us to disclose ALS spectroscopic markers related to protein and lipid alterations, as well as to a reduction of the phenylalanine level, in comparison with HCs. Our findings show that vibrational spectroscopy is a new potential ALS diagnostic approach and indicate that tears are a reliable and non-invasive source of ALS biomarkers. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Tear-Based_Vibrational_Spectroscopy_Applied_to_Amyotrophic_Lateral_Sclerosis/17203861Test |
| DOI: |
10.1021/acs.analchem.1c02546.s001 |
| الإتاحة: |
https://doi.org/10.1021/acs.analchem.1c02546.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.19847485 |
| قاعدة البيانات: |
BASE |
