دورية أكاديمية
FRAX486, a PAK inhibitor, overcomes ABCB1-mediated multidrug resistance in breast cancer cells
| العنوان: | FRAX486, a PAK inhibitor, overcomes ABCB1-mediated multidrug resistance in breast cancer cells |
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| المؤلفون: | Meng Zhang, Xiaoqi Zeng, Meiling She, Xingduo Dong, Jun Chen, Qingquan Xiong, Guobin Qiu, Shuyi Yang, Xiangqi Li, Guanghui Ren |
| المصدر: | Brazilian Journal of Medical and Biological Research, Vol 57 (2024) |
| بيانات النشر: | Associação Brasileira de Divulgação Científica, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | FRAX486, p21-activated kinase (PAK) inhibitor, Multidrug resistance, ABC transporter, ABCB1, Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | The overexpression of P-glycoprotein (P-gp/ABCB1) is a leading cause of multidrug resistance (MDR). Hence, it is crucial to discover effective pharmaceuticals that counteract ABCB1-mediated multidrug resistance. FRAX486 is a p21-activated kinase (PAK) inhibitor. The objective of this study was to investigate whether FRAX486 can reverse ABCB1-mediated multidrug resistance, while also exploring its mechanism of action. The CCK8 assay demonstrated that FRAX486 significantly reversed ABCB1-mediated multidrug resistance. Furthermore, western blotting and immunofluorescence experiments revealed that FRAX486 had no impact on expression level and intracellular localization of ABCB1. Notably, FRAX486 was found to enhance intracellular drug accumulation and reduce efflux, resulting in the reversal of multidrug resistance. Docking analysis also indicated a strong affinity between FRAX486 and ABCB1. This study highlights the ability of FRAX486 to reverse ABCB1-mediated multidrug resistance and provides valuable insights for its clinical application. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1414-431X 1414-431x |
| العلاقة: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2024000100654&lng=en&tlng=enTest; http://www.scielo.br/pdf/bjmbr/v57/1414-431X-bjmbr-57-e13357.pdfTest; https://doaj.org/toc/1414-431XTest |
| DOI: | 10.1590/1414-431x2024e13357 |
| الوصول الحر: | https://doaj.org/article/2822698d56194bf1845e291b61ac846cTest |
| رقم الانضمام: | edsdoj.2822698d56194bf1845e291b61ac846c |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1414431X 1414431x |
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| DOI: | 10.1590/1414-431x2024e13357 |