Perturbed development of calb2b expressing dI6 interneurons and motor neurons underlies locomotor defects observed in calretinin knock-down zebrafish larvae
العنوان: | Perturbed development of calb2b expressing dI6 interneurons and motor neurons underlies locomotor defects observed in calretinin knock-down zebrafish larvae |
---|---|
المؤلفون: | Iglesias Gonzalez, Ana Belen, Koning, Harmen Kornelis, 1991, Tuz-Sasik, Melek Umay, van Osselen, Ilse, Manuel, Remy, Boije, Henrik |
المصدر: | Developmental Biology. 508:77-87 |
مصطلحات موضوعية: | dmrt3a, Spinal cord, Evx2, Calcium binding protein, Locomotor network |
الوصف: | Calcium binding proteins are essential for neural development and cellular activity. Calretinin, encoded by calb2a and calb2b, plays a role during early zebrafish development and has been proposed as a marker for distinct neuronal populations within the locomotor network. We generated a calb2b:hs:eGFP transgenic reporter line to characterize calretinin expressing cells in the developing spinal cord and describe morphological and behavioral defects in calretinin knock-down larvae. eGFP was detected in primary and secondary motor neurons, as well as in dI6 and V0v interneurons. Knock-down of calretinin lead to disturbed development of motor neurons and dI6 interneurons, revealing a crucial role during early development of the locomotor network. Primary motor neurons showed delayed axon outgrowth and the distinct inhibitory CoLo neurons, originating from the dI6 lineage, were absent. These observations explain the locomotor defects we observed in calretinin knock-down animals where the velocity, acceleration and coordination were affected during escapes. Altogether, our analysis suggests an essential role for calretinin during the development of the circuits regulating escape responses and fast movements within the locomotor network. |
وصف الملف: | electronic |
الوصول الحر: | https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-528186Test https://doi.org/10.1016/j.ydbio.2024.01.001Test https://uu.diva-portal.org/smash/get/diva2:1858728/FULLTEXT01.pdfTest |
قاعدة البيانات: | SwePub |
تدمد: | 00121606 1095564X |
---|---|
DOI: | 10.1016/j.ydbio.2024.01.001 |