دورية أكاديمية
The use of a conformational cathepsin D-derived epitope for vaccine development against Schistosoma mansoni
| العنوان: | The use of a conformational cathepsin D-derived epitope for vaccine development against Schistosoma mansoni |
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| المؤلفون: | Ahmad Fuaad, Abdullah A. H., Roubille, Romain, Pearson, Mark S., Pickering, Darren A., Loukas, Alex C., Skwarczynski, Mariusz, Toth, Istvan |
| بيانات النشر: | Pergamon Press |
| سنة النشر: | 2015 |
| المجموعة: | The University of Queensland: UQ eSpace |
| مصطلحات موضوعية: | Nanoparticles, Subunit peptide-based vaccine, Lipid core peptide, Epitope modification, Peptide conformation, Self-assembly, Schistosomiasis, Schistosoma mansoni, 2700 Medicine, 1303 Biochemistry, 1308 Clinical Biochemistry, 1312 Molecular Biology, 1313 Molecular Medicine, 1605 Organic Chemistry, 3002 Drug Discovery, 3003 Pharmaceutical Science |
| الوصف: | Schistosomiasis is caused by the infection from Schistosoma species. Among these, Schistosoma mansoni is one of the major species that infects millions of people worldwide. The use of praziquantel is effective in clearing the infestation but treatment of a large and widespread population in endemic areas is unsustainable. Thus, synergistic approach of using drug and vaccination can serve as an alternative to the current treatment. In this study, we have developed vaccine candidates that composed of three components: a B-cell epitope derived from S. mansoni cathepsin D protein (Sm-CatD) flanked by GCN4 helix promoting peptide; a promiscuous T-helper epitope (P25); and a lipid core peptide system, in attempt to develop self-adjuvanting vaccine candidates against the schistosome. Physicochemical properties of the vaccine candidates were analysed and antibodies to each construct were raised in BALB/c mice. The vaccine candidates were able to self-assemble into particles that induced high titres of IgG without the use of additional adjuvant. The antibody levels were comparable to that induced by peptide formulated with strong but toxic Freund's adjuvant. The integration of a GCN4 sequence induced the helical conformation of the epitope, while the addition of the T helper peptide was very effective in inducing consistent IgG-specific antibodies response amongst mice. These findings are particularly encouraging for the development of efficient and immunogenic vaccine against schistosomiasis. (C) 2015 Elsevier Ltd. All rights reserved. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0968-0896 1464-3391 |
| العلاقة: | orcid:0000-0001-7257-807X; orcid:0000-0002-4572-397X; 496600 |
| الإتاحة: | https://doi.org/10.1016/j.bmc.2015.01.033Test https://espace.library.uq.edu.au/view/UQ:352013Test |
| رقم الانضمام: | edsbas.2B8C7AAE |
| قاعدة البيانات: | BASE |
| تدمد: | 09680896 14643391 |
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