In vitro and in vivo comparative and competitive activity-based protein profiling of GH29 alpha-L-fucosidases
| العنوان: | In vitro and in vivo comparative and competitive activity-based protein profiling of GH29 alpha-L-fucosidases |
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| المؤلفون: | Adrianus M. C. H. van den Nieuwendijk, Daniel Mink, Veronica Coco Rohde, Gijsbert A. van der Marel, Jianbing Jiang, Daniel W. Wright, Bogdan I. Florea, Johannes M. F. G. Aerts, Gideon J. Davies, Wouter W. Kallemeijn, Hans van den Elst, Wilma E. Donker-Koopman, Martin Schürmann, Nan Li, Rolf G. Boot, Saskia Scheij, Marri Verhoek, Jeroen D. C. Codée, Herman S. Overkleeft, Elisa Colomina Folch |
| المساهمون: | Other departments, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Medical Biochemistry, ACS - Amsterdam Cardiovascular Sciences |
| المصدر: | CHEMICAL SCIENCE, 6(5), 2782-2789. Royal Society of Chemistry Chemical Science, 6(5), 2782-2789 |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | biology, genetic structures, 010405 organic chemistry, Stereochemistry, Chemistry, Activity-based proteomics, Active site, Stereoisomerism, General Chemistry, 010402 general chemistry, medicine.disease, biology.organism_classification, 01 natural sciences, Fucosidosis, In vitro, 0104 chemical sciences, 3. Good health, Biochemistry, In vivo, Hydrolase, medicine, biology.protein, Bacteria |
| الوصف: | GH29 alpha-L-fucosidases catalyze the hydrolysis of alpha-L-fucosidic linkages. Deficiency in human lysosomal alpha-L-fucosidase (FUCA1) leads to the recessively inherited disorder, fucosidosis. Herein we describe the development of fucopyranose-configured cyclophellitol aziridines as activity-based probes (ABPs) for selective in vitro and in vivo labeling of GH29 alpha-L-fucosidases from bacteria, mice and man. Crystallographic analysis on bacterial alpha-L-fucosidase confirms that the ABPs act by covalent modification of the active site nucleophile. Competitive activity-based protein profiling identified L-fuconojirimycin as the single GH29 alpha-L-fucosidase inhibitor from eight configurational isomers |
| اللغة: | English |
| تدمد: | 2041-6539 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9c1b0655d1799327f935b9d7fee32e0Test https://doi.org/10.1039/c4sc03739aTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c9c1b0655d1799327f935b9d7fee32e0 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20416539 |
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