التفاصيل البيبلوغرافية
| العنوان: |
Cardiovascular complications - 2 |
| المؤلفون: |
Molostvov, Guerman, Docrat, Naadiya, Omotoye, Samuel, Fletcher, Simon, Bland, Rosemary, Zehnder, Daniel, Luksha, Leanid, Luksha, Natallia, Carrero, Juan Jesus, Hammarqvist, Folke, Stenvinkel, Peter, Kublickiene, Karolina, Schlieper, Georg, Neizel, Mirja, Krüger, Thilo, Floege, Jürgen, Kelm, Malte, Kühl, Harald, Westenfeld, Ralf, Mistrik, Erik, Dusilova-Sulkova, Sylvie, Blaha, Vladimir, Sobotka, Lubos, Selim, Gjulsen, Stojceva-Taneva, Olivera, Zafirova-Ivanovska, Beti, Trojacanec-Piponska, Sonja, Gelev, Saso, Nikolov, Igor, Stojcev, Nikola, Dzekova, Pavlina, Rambabova-Busletic, Irena, Asani, Arben, Sikole, Aleksandar, Chen, Yaxi, Varghese, Zac, Huang, Ailong, Yang, Fen, Lei, Han, Moorhead, John, Powis, Stephen, Ruan, Xiong, David, Sascha, Kümpers, Philipp, Lukasz, Alexander, Fliser, Danilo, Kliem, Volker, Haller, Hermann, Kielstein, Jan T., Malavade, Tushar, Kansara, Manthan, Hegde, Umapati, Gohel, Kalpesh, Gang, Sishir, Mukhopadhyay, Banibrata, Lele, Suhas, Rajapurkar, Mohan, Lambers Heerspink, H.J., Ninomiya, T., Zoungas, S., Jardine, M.J., Roberts, M.A., Cass, A., Perkovic, V., El-Shaarawy, Abdel-Bassit, El-Saeed, Ashraf, El-Kheshen, Jamal, Kuo, Ko-Lin, Hung, Szu-Chun, Tarng, Der-Cherng, Jourde Chiche, Noemie, Dou, Laetitia, Sabatier, Florence, Calaf, Raymond, Cerini, Claire, Robert, Stephane, Camoin Jau, Laurence, Sampol, Jose, Charpiot, Philippe, Berland, Yvon, Argiles, Angel, Dignat George, Francoise, Brunet, Philippe, van Eps, Carolyn, Jeffries, Leanne, Haluska, Brian, Hawley, Carmel, Jeffries, Janine, Johnson, David, Campbell, Scott, Isbel, Nicole, Mudge, David, Marwick, Thomas, Mendoza, F.J., Guerrero, F., Lopez, I., Aguilera-Tejero, E., Rodriguez, M., Tankovics, Anna, Fischer, Christoph, Wilde, Adelheid, Boenisch, Olaf, Zickler, Daniel, Boehme, Michael, Schindler, Ralf, Aoki, Asuka, Kikuchi, Kan, Tsukada, Misao, Miwa, Naoko, Kimata, Naoki, Akiba, Takashi, Nitta, Kosaku, Esposito, Pasquale, Libetta, Carmelo, Cosmai, Laura, Portalupi, Valentina, Gregorini, Marilena, Rampino, Teresa, Dal Canton, Antonio, Patel, Rajan, Mark, Patrick, Cobbe, Stuart, Dargie, Henry, Jardine, Alan G., Hernandes, Fabiana R., Barreto, Fellype C., Barreto, Daniela V., Moyses, Rosa Maria A., Jorgetti, Vanda, Neves, Kátia R., Canziani, Maria Eugênia F., de Carvalho, Aluizio B., Wystrychowski, Grzegorz, Zukowska-Szczechowska, Ewa, Grzeszczak, Wladyslaw, Kirmizis, Dimitrios, Papagianni, Aikaterini, Belechri, Anna-Maria, Skoura, Lemonia, Samara, Magda, Efstratiadis, Georgios, Alexopoulos, Efstathios, Memmos, Dimitrios, Mortazavi Najafabadi, Mojgan, Taheri, Sharam, Seyrafian, Shiva, Alipoor, Zeynab, Karimi, Shirin, Poormoghadas, Ali, Ghassami, Maryam, Dovas, Spyros, Bantis, Christos, Zafeiriadou, Efthimia, Tsinoglou, Konstantinos, Memmos, Dimitirios, Chabu, James, Naicker, Saraladevi, Manga, Pravin, Woodiwiss, Angela, Norton, Gavin, Wang, Tao, Cheng, Li-Tao, Zhang, Ai-Hua, Zheng, Dan-Xia, Han, Qing-Feng, Yildiz, Alaattin, Oflaz, Huseyin, Yazici, Halil, Pusuroglu, Hamdi, Tepe, Savas, Memisoglu, Esat, Akkaya, Vakur, Basci, Ali, Fang, Te-Chao, Shen, Tsu-Wang, Wang, Chih-Hsien, Lai, Yu-Hsien, Hsu, Bang-Gee, Murakami, Tomoyuki, Iwamoto, Tamio, Ono, Shuji, Yanagi, Mai, Koguchi, Naoaki, Kawata, Seiichi, Shibata, Kazuhiko, Satta, Hidehisa, Kuji, Tadashi, Ikee, Ryota, Honda, Kenjiro, Ishioka, Kunihiro, Oka, Machiko, Maesato, Kyoko, Moriya, Hidekazu, Hidaka, Sumi, Ohtake, Takayasu, Kobayashi, Shuzo, Severi, Stefano, Pogliani, Daniela, Fantini, Giulia, Fabbrini, Paolo, Galbiati, Eleonora, Bonforte, Giuseppe, Vincenti, Antonio, Stella, Andrea, Genovesi, Simonetta, Oyake, Nobuyuki, Suzuki, Keiko, Tanabe, Kazuaki, Shimada, Toshio, Georgievska-Ismail, Ljubica, Srbinovska-Kostovska, Elizabeta, Moriyama, Yukie, Eriguchi, Rieko, Sato, Yuzuru, Batmazoglu, Mehmet, Dursun, Belda, Yagci, Baki, Cakmak, Pinar, Rota, Simin, Akdag, Beyza, Chiarelli, Giusy, Beaulieu, Monica, Brin, Genevieve, Singh, Suneet, Jamal, Abeed, Taylor, Paul, Levin, Adeera, An, Wonsuk, Kim, Seong-Eun, Kim, Ki-Hyun, Yoo, Hyun-Seung, Son, Sung-Hyun, Korotvicka, Milan, Eiselt, Jaromir, Malanova, Lada, Trefil, Ladislav, Rajdl, Daniel, Vostry, Michal, Racek, Jaroslav, Murasawa, Tsuneo, Sakai, Yukinao, Ohtsuka, Tomoyuki, Ohno, Dai, Amitani, Ken-ichi, Ono, Takuya, Munakata, Kazuo, Grieve, Alison, Ansari, Asif, Smith, Graham, Smith, William, Anwar, Shahid, Nicholls, Kathy, Jagga, Asim, Zaluska, Wojciech, Szeliga-Król, Jolanta, Bednarek-Skublewska, Anna, Ksiazek, Andrzej, Karasavvidou, Despina, Kalaitzidis, Rigas, Dounousi, Eva, Katopodis, Kostas, Pelidou, Sygliti-Errieta, Zikou, Xanthi, Siamopoulos, Kostas, Yamada, Kazuhiro, Fujimoto, Shouichi, Fukudome, Keiichi, Tatsumoto, Mariko, Sato, Yuji, Hisanaga, Shuichi, Ochiai, Hideyuki, Kitamura, Kazuo, Dimitriadis, Chrysostomos, Bellechri, Anna-Maria, Liakou, Eleni, Hayashi, Terumasa, Sasaki, Koichi, Yasuda, Keiko, Obi, Yoshitsugu, Takeda, Yoshihiro, Nagai, Yoshiyuki, Kimura, Tomonori, Ono, Syuji, Kawata, Seichi, Saka, Sanae, Ando, Daisaku, Hirawa, Shinji, Yasuda, Gen, Toya, Yoshiyuki, Umemura, Satoshi, Vareesangthip, Kriengsak, Laouthaiwattana, Piyaporn, Ongajyooth, Leena, Lee, Kang Wook, Jung, Ji-Yoon, Kim, Eui-Sic, Chung, Sarah, Choi, Dae-Eun, Na, Ki-Ryang, Shin, Young-Tai, Mircescu, G., Sintimbreanu, C., Rus, E., Barbulescu, C., Klein, C., Martinescu, R., Petrescu, L., Zugravu, A., Stancu, S., Covic, A., Marn Pernat, Andreja, Buturovic-Ponikvar, Jadranka, Ponikvar, Rafael |
| بيانات النشر: |
Oxford University Press |
| سنة النشر: |
2010 |
| المجموعة: |
HighWire Press (Stanford University) |
| مصطلحات موضوعية: |
Sunday |
| الوصف: |
INTRODUCTION AND AIMS: Progressive arterial calcification is a major cause of cardiovascular mortality in patients with chronic kidney disease. We have recently demonstrated expression of the calcium-sensing receptor (CaSR) in human aortic smooth muscle cells (HAoSMC) and human arteries and demonstrated a correlation between CaSR expression and medial calcification. Here we investigated the role of SMC phenotype, pro-inflammatory factors and pulsatile strain in the development and progression of vascular calcification and CaSR expression. METHODS: HAoSMC were cultured for up to 4 weeks in the presence of 5mM b-glycerophosphate (b-GP) or 20ng/ml TNF-a alone or in combination. CaSR and a-actin (SMC marker) protein expression was analysed using Western blot. Cells were stained with alizarin red to assess calcification. In addition, cells were cultured under a cyclic biaxial strain (7% stretch, 30 cycles/min) using Flexcell apparatus and collagen I coated dishes for up to 14 days. Statistical analysis was performed using one-way ANOVA followed by Tukey's multiple comparison tests. RESULTS: Long-term culture of HAoSMC resulted in a gradual decline in smooth muscle a-actin expression, which decreased by 35% (p<0.05) after 2 weeks and >60% (p<0.01) after 4 weeks of culture. This was accompanied by a 45% reduction in CaSR protein expression (p<0.05). SMC a-actin expression decreased further following incubation for 4 weeks with b-GP (79%, p<0.05) or TNF-a (65%, p<0.05). This was not further altered by co-treatment with b-GP and TNF-a. No further change of CaSR protein expression was observed following incubation with b-GP or TNF-a. Alizarin red staining revealed larger areas of calcification in b-GP and TNF-a treated cultures after 4 weeks of treatment when compared to control cells; however, this increase was not significant. Culture of HAoSMC under cyclic strain produced a significant up-regulation of a-actin expression by day 7 and 10 (maximum 23%, p<0.05) compared to control static cultures. ... |
| نوع الوثيقة: |
text |
| وصف الملف: |
text/html |
| اللغة: |
English |
| العلاقة: |
http://ckj.oxfordjournals.org/cgi/content/short/2/suppl_2/ii1985Test; http://dx.doi.org/10.1093/ndtplus/2.s2.30Test |
| DOI: |
10.1093/ndtplus/2.s2.30 |
| الإتاحة: |
https://doi.org/10.1093/ndtplus/2.s2.30Test
http://ckj.oxfordjournals.org/cgi/content/short/2/suppl_2/ii1985Test |
| حقوق: |
Copyright (C) 2010, European Renal Association - European Dialysis and Transplant Assoc |
| رقم الانضمام: |
edsbas.96BD5479 |
| قاعدة البيانات: |
BASE |
