Functional T-Cell Deficiency in Adolescents Who Experience Serogroup C Meningococcal Disease despite Receiving the Meningococcal Serogroup C Conjugate Vaccine
| العنوان: | Functional T-Cell Deficiency in Adolescents Who Experience Serogroup C Meningococcal Disease despite Receiving the Meningococcal Serogroup C Conjugate Vaccine |
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| المؤلفون: | Mary Ramsay, Andrew Lees, M.W. McKendrick, Robert C. Read, Ed Kacsmarski, Elizabeth L. Miller, Ray Borrow, Jennifer Carlring, Rachel A. Foster, Andrew W. Heath |
| المصدر: | Clinical and Vaccine Immunology. 17:1104-1110 |
| بيانات النشر: | American Society for Microbiology, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | CD4-Positive T-Lymphocytes, Microbiology (medical), Adolescent, T-Lymphocytes, Clinical Biochemistry, Immunology, Meningococcal Vaccines, Meningococcal vaccine, Meningitis, Meningococcal, Lymphocyte Activation, Meningococcal disease, Peripheral blood mononuclear cell, Conjugate vaccine, medicine, Humans, Immunology and Allergy, Treatment Failure, Cells, Cultured, Cell Proliferation, business.industry, Toxoid, Bacterial polysaccharide, Vaccine Research, medicine.disease, Virology, Vaccination, business, Vaccine failure |
| الوصف: | Some individuals have experienced meningococcal disease despite receiving the meningococcal serogroup C conjugate (MCC) vaccine in adolescence. We sought to determine whether this is due to subclinical functional B- or T-cell immunodeficiency. Of 53 vaccine failures identified by enhanced surveillance of England and Wales from 1999 to 2004, 15 received MCC vaccine in adolescence, 9 of whom were recruited 2 to 6 years following convalescence from meningococcal disease. Their peripheral blood mononuclear cells (PBMCs) were incubated with polyclonal activators designed to mimic T-cell-independent B-cell stimulation by bacterial polysaccharides and the T-cell stimulation provided by the protein component of the conjugate vaccine. Subsequent proliferation and activation of T and B lymphocytes were measured, along with T-cell help to B cells. Compared to age-, sex-, geographically, and ethnicity-matched controls, CD4 T-cell proliferation rates in response to both anti-CD3 (T-cell receptor [TCR]) stimulation and anti-CD3 in the presence of B cells activated through anti-IgD conjugated to dextran (α-δ-dex) were lower in PBMCs derived from vaccine failures (P= 0.044 andP= 0.029, respectively). There was reduced CD4 cell activation of the patient cells compared to controls following stimulation by CD3 (P= 0.048). B-cell activation during incubation of PBMCs with the T-cell stimuli, anti-CD3 (P= 0.044), or anti-CD3 plus anti-CD28 (P= 0.018) was relatively impaired in patients. Anti-tetanus toxoid IgG concentrations were lower in the vaccine failure group (P= 0.0385). There was a relative defect of T-cell responsiveness to T-cell-dependent antigen stimulation in MCC vaccine failures, which was manifested in reduced T-cell help to B cells. |
| تدمد: | 1556-679X 1556-6811 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd5370b85896f5852e36732a617a8e71Test https://doi.org/10.1128/cvi.00481-09Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....cd5370b85896f5852e36732a617a8e71 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1556679X 15566811 |
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