دورية أكاديمية
An engineered anti-idiotypic antibody-derived killer peptide (KP) early activates swine inflammatory monocytes, CD3+CD16+ natural killer T cells and CD4+CD8α+ double positive CD8β+ cytotoxic T lymphocytes associated with TNF-α and IFN-γ secretion
| العنوان: | An engineered anti-idiotypic antibody-derived killer peptide (KP) early activates swine inflammatory monocytes, CD3+CD16+ natural killer T cells and CD4+CD8α+ double positive CD8β+ cytotoxic T lymphocytes associated with TNF-α and IFN-γ secretion |
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| المؤلفون: | Ferrari L., Martelli P., Saleri R., De Angelis E., Ferrarini G., Cavalli V., Passeri B., Bazzoli G., Ogno G., Magliani W., Borghetti P. |
| المساهمون: | Ferrari, L., Martelli, P., Saleri, R., De Angelis, E., Ferrarini, G., Cavalli, V., Passeri, B., Bazzoli, G., Ogno, G., Magliani, W., Borghetti, P. |
| سنة النشر: | 2020 |
| المجموعة: | Università di Parma: CINECA IRIS |
| مصطلحات موضوعية: | Cytokine, Double positive cytotoxic T lymphocytes (DP CTL), IFN-γ secreting cell, Killer peptide (KP), Natural killer (NK) T cell, Porcine circovirus type 2 (PCV2), Porcine reproductive and respiratory syndrome virus (PRRSV), Pro-inflammatory monocyte, TNF-α, Viral cross-reactivity |
| الوصف: | This study evaluated the early modulation of the phenotype and cytokine secretion in swine immune cells treated with an engineered killer peptide (KP) based on an anti-idiotypic antibody functionally mimicking a yeast killer toxin. The influence of KP on specific immunity was investigated using porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) as ex vivo antigens. Peripheral blood mononuclear cells (PBMC) from healthy pigs were stimulated with KP and with a scramble peptide for 20 min, 1, 4 and 20 h or kept unstimulated. The cells were analyzed using flow cytometry and ELISA. The same time-periods were used for KP pre-incubation/co-incubation to determine the effect on virus-recalled interferon-gamma (IFN-γ) secreting cell (SC) frequencies and single cell IFN-γ productivity using ELISPOT. KP induced an early dose-dependent shift to pro-inflammatory CD172α+CD14+high monocytes and an increase of CD3+CD16+ natural killer (NK) T cells. KP triggered CD8α and CD8β expression on classical CD4−CD8αβ+ cytotoxic T lymphocytes (CTL) and double positive (DP) CD4+CD8α+ Th memory cells (CD4+CD8α+low CD8β+low). A fraction of DP cells also expressed high levels of CD8α. The two identified DP CD4+CD8α+high CD8β+low/+high CTL subsets were associated with tumor necrosis factor alpha (TNF-α) and IFN-γ secretion. KP markedly boosted the reactivity and cross-reactivity of PRRSV type-1- and PCV2b-specific IFN-γ SC. The results indicate the efficacy of KP in stimulating Th1-biased immunomodulation and support studies of KP as an immunomodulator or vaccine adjuvant. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/WOS:000572830100012; volume:72; firstpage:1; lastpage:15; numberofpages:15; journal:COMPARATIVE IMMUNOLOGY, MICROBIOLOGY AND INFECTIOUS DISEASES; http://hdl.handle.net/11381/2900169Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85089002527 |
| DOI: | 10.1016/j.cimid.2020.101523 |
| الإتاحة: | https://doi.org/10.1016/j.cimid.2020.101523Test http://hdl.handle.net/11381/2900169Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.9024618D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.cimid.2020.101523 |
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