دورية أكاديمية
GLP-1 Receptor Signaling Has Different Effects on the Perikarya and Axons of the Hypophysiotropic Thyrotropin-Releasing Hormone Synthesizing Neurons in Male Mice
| العنوان: | GLP-1 Receptor Signaling Has Different Effects on the Perikarya and Axons of the Hypophysiotropic Thyrotropin-Releasing Hormone Synthesizing Neurons in Male Mice |
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| المؤلفون: | Ruska, Yvette Magdolna, Péterfi, Zoltán Attila, Stiftné Szilvásy-Szabó, Anett, Kővári, Dóra, Hrabovszky, Erik, Dorogházi, Beáta Vanessza, Gereben, Balázs, Tóth, Blanka, Matziari, Magdalini, Wittmann, Gábor, Fekete, Csaba |
| سنة النشر: | 2024 |
| المجموعة: | MTAK: REAL (Library and Information Centre of the Hungarian Academy of Sciences / Magyar Tudományos Akadémia Könyvtár és Információs Központ) |
| مصطلحات موضوعية: | QH301 Biology / biológia, R1 Medicine (General) / orvostudomány általában |
| الوصف: | Background: Glucagon-like peptide 1 (GLP-1) is involved in the regulation of energy and glucose homeostasis. As GLP-1 has similar effects on the energy homeostasis as the hypophysiotropic thyrotropin-releasing hormone (TRH) neurons that regulate the hypothalamic-pituitary-thyroid (HPT) axis, we raised the possibility that the TRH neurons are involved in the mediation of the effects of GLP-1. Therefore, the relationship and interaction of the GLP-1 system and the TRH neurons of the hypothalamic paraventricular nucleus (PVN) were studied.Methods: To examine the anatomical and functional relationship of TRH neurons and the GLP-1 system in the PVN, immunocytochemistry, in situ hybridization, in vitro patch-clamp electrophysiology, metabolic phenotyping, and explant experiments were performed.Results: Our data demonstrate that the TRH neurons of the PVN are innervated by GLP-1 producing neurons and express the GLP-1 receptor (GLP-1R). However, not only do the GLP-1-innervated TRH neurons express GLP-1R but the receptor is also present in the axons of the hypophysiotropic TRH neurons in the blood-brain barrier free median eminence (ME) suggesting that peripherally derived GLP-1 may also influence the TRH neurons. In vitro, GLP-1 increased the firing rate of TRH neurons and depolarized them. In addition, GLP-1 directly stimulated the GABAergic input of a population of TRH neurons. Furthermore, GLP-1 inhibited the release of TRH from the hypophysiotropic axons in the ME. In vivo, peripheral GLP-1R agonist administration markedly inhibited the food intake and the energy expenditure, but had no effect on the TRH expression in the PVN and resulted in lower circulating free T4 levels.Conclusions: Our results indicate that GLP-1R activation has a direct stimulatory effect on TRH neurons in the PVN, but the activation of GLP-1R may also inhibit TRH neurons by facilitating their inhibitory inputs or by inhibiting the axon terminals of these cells in the ME. The innervation of TRH neurons by GLP-1 neurons suggests that TRH ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | text |
| اللغة: | English |
| العلاقة: | https://real.mtak.hu/191246/1/GLP-1receptorsignalinghasdifferenteffectsontheperikaryaandaxonsofthe.pdfTest; Ruska, Yvette Magdolna and Péterfi, Zoltán Attila and Stiftné Szilvásy-Szabó, Anett and Kővári, Dóra and Hrabovszky, Erik and Dorogházi, Beáta Vanessza and Gereben, Balázs and Tóth, Blanka and Matziari, Magdalini and Wittmann, Gábor and Fekete, Csaba (2024) GLP-1 Receptor Signaling Has Different Effects on the Perikarya and Axons of the Hypophysiotropic Thyrotropin-Releasing Hormone Synthesizing Neurons in Male Mice. THYROID, 34 (2). pp. 252-260. ISSN 1050-7256 |
| DOI: | 10.1089/thy.2023.0284 |
| الإتاحة: | https://doi.org/10.1089/thy.2023.0284Test https://real.mtak.hu/191246Test/ https://real.mtak.hu/191246/1/GLP-1receptorsignalinghasdifferenteffectsontheperikaryaandaxonsofthe.pdfTest |
| رقم الانضمام: | edsbas.B298DC37 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1089/thy.2023.0284 |
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