التفاصيل البيبلوغرافية
| العنوان: |
Development and validation of a high-performance liquid chromatography method using diode array detection for the simultaneous quantification of aripiprazole and dehydro-aripiprazole in human plasma |
| المؤلفون: |
Lancelin, Frédérique1 l.lancelin@ch-sainte-anne.fr, Djebrani, Kayssa2, Tabaouti, Khalid1, Kraoul, Linda1, Brovedani, Sophie1, Paubel, Pascal2, Piketty, Marie-Liesse1 |
| المصدر: |
Journal of Chromatography B: Analytical Technologies in the Biomedical & Life Sciences. May2008, Vol. 867 Issue 1, p15-19. 5p. |
| مصطلحات موضوعية: |
*HIGH performance liquid chromatography, *LIQUID chromatography, *DIODES, *CHROMATOGRAPHIC analysis, *CALIBRATION |
| مستخلص: |
Abstract: A high-performance liquid chromatography method with diode array detection (HPLC-DAD) was developed for quantification of aripiprazole and dehydro-aripiprazole, in human plasma. After a simple liquid–liquid extraction, chromatographic separation was carried out on a C18 reversed-phase column, using an ammonium buffer–acetonitrile mobile phase (40:60, v/v). The total run time was only 7min at a flow-rate of 1.0ml/min. The precision values were less than 12% and the accuracy values were ranging from 98 to 113% and the lower limit of quantification was 2ng/ml for both compounds. Calibration curves were linear over a range of 2–1000ng/ml. The mean trough plasma concentrations in patients treated with aripiprazole were 157 and 29ng/ml for aripiprazole and dehydro-aripiprazole, respectively. [Copyright &y& Elsevier] |
| قاعدة البيانات: |
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