Identification of reference genes for quantitative RT-PCR in ascending aortic aneurysms
| العنوان: | Identification of reference genes for quantitative RT-PCR in ascending aortic aneurysms |
|---|---|
| المؤلفون: | Hilja Perttunen, Dominic Henn, Doris Bandner-Risch, Wolfram Schmied, Noela Rodriguez-Losada, Hans-Joachim Schäfers, Francisco C. Ceballos, Carlos Porras |
| المساهمون: | [Henn,D, Bandner-Risch,D, Perttunen,H, Schmied,W, Schäfers,H-J] Department of Thoracic and Cardiovascular Surgery, Saarland University Hospital, Homburg/Saar, Germany. [Porras,C] Department of Cardiac Surgery, Hospital Universitario Virgen de la Victoria de Málaga, Spain. [Ceballos,F] Department of Genetics, Faculty of Biology, University of Santiago de Compostela, Spain. [Rodríguez-Losada,N] Foundation IMABIS, Carlos Haya University Hospital, Avenida Carlos Haya, Malaga, Spain. |
| المصدر: | PLoS ONE PLoS ONE, Vol 8, Iss 1, p e54132 (2013) |
| بيانات النشر: | Public Library of Science, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Aortic valve, Male, Pathology, Cardiothoracic Surgery, Anatomy and Physiology, Valvular Disease, Hemodynamics, Gene Expression, Cardiovascular, Cardiovascular System, Phenomena and Processes::Genetic Phenomena::Genetic Processes::Gene Expression [Medical Subject Headings], Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Genetic Association Studies [Medical Subject Headings], Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Primates::Haplorhini::Catarrhini::Hominidae::Humans [Medical Subject Headings], Aortic aneurysm, Reference genes, Factor 2B Eucariótico de Iniciación, Masculino, Aorta, Diseases::Cardiovascular Diseases::Vascular Diseases::Aneurysm::Aortic Aneurysm [Medical Subject Headings], Multidisciplinary, Cardiovascular Surgery, Reverse Transcriptase Polymerase Chain Reaction, Adulto, Femenino, Nuclear Proteins, Middle Aged, Aortic Aneurysm, Humanos, Analytical, Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Genetic Techniques::Nucleic Acid Amplification Techniques::Polymerase Chain Reaction::Reverse Transcriptase Polymerase Chain Reaction [Medical Subject Headings], Eukaryotic Initiation Factor-2B, medicine.anatomical_structure, Hypertension, Cardiology, cardiovascular system, Medicine, Cyclophilin A, Dilatation, Pathologic, Research Article, Adult, medicine.medical_specialty, Expresión Génica, Science, Check Tags::Male [Medical Subject Headings], Biology, Receptor, Angiotensin, Type 1, Molecular Genetics, Diseases::Cardiovascular Diseases::Heart Diseases::Heart Valve Diseases [Medical Subject Headings], Diseases::Cardiovascular Diseases::Vascular Diseases::Hypertension [Medical Subject Headings], Vascular Biology, Anatomy::Tissues [Medical Subject Headings], medicine.artery, Internal medicine, Ascending aorta, Aneurisma de la Aorta, Enfermedades de las Válvulas Cardíacas, Hipertensión, medicine, Genetics, Named Groups::Persons::Age Groups::Adult [Medical Subject Headings], Humans, Angiotensin II receptor type 1, Human Genetics, Chemicals and Drugs::Amino Acids, Peptides, and Proteins::Peptides::Intracellular Signaling Peptides and Proteins::GTP-Binding Protein Regulators::Guanine Nucleotide Exchange Factors::Eukaryotic Initiation Factor-2B [Medical Subject Headings], medicine.disease, Reacción en Cadena de la Polimerasa de Transcriptasa Inversa, Tejidos, Check Tags::Female [Medical Subject Headings], Genetics of Disease, Cardiovascular Anatomy, Surgery, Unicuspid, Estudios de Asociación Genética, Transcription Factors |
| الوصف: | Journal Article; Hypertension and congenital aortic valve malformations are frequent causes of ascending aortic aneurysms. The molecular mechanisms of aneurysm formation under these circumstances are not well understood. Reference genes for gene activity studies in aortic tissue that are not influenced by aortic valve morphology and its hemodynamic consequences, aortic dilatation, hypertension, or antihypertensive medication are not available so far. This study determines genes in ascending aortic tissue that are independent of these parameters. Tissue specimens from dilated and undilated ascending aortas were obtained from 60 patients (age ≤70 years) with different morphologies of the aortic valve (tricuspid undilated n = 24, dilated n = 11; bicuspid undilated n = 6, dilated n = 15; unicuspid dilated n = 4). Of the studied individuals, 36 had hypertension, and 31 received ACE inhibitors or AT1 receptor antagonists. The specimens were obtained intraoperatively from the wall of the ascending aorta. We analyzed the expression levels of 32 candidate reference genes by quantitative RT-PCR (RT-qPCR). Differential expression levels were assessed by parametric statistics. The expression analysis of these 32 genes by RT-qPCR showed that EIF2B1, ELF1, and PPIA remained constant in their expression levels in the different specimen groups, thus being insensitive to aortic valve morphology, aortic dilatation, hypertension, and medication with ACE inhibitors or AT1 receptor antagonists. Unlike many other commonly used reference genes, the genes EIF2B1, ELF1, and PPIA are neither confounded by aortic comorbidities nor by antihypertensive medication and therefore are most suitable for gene expression analysis of ascending aortic tissue. Yes |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::697ecacef49f393f4e437f067a29d2cfTest https://hdl.handle.net/10668/877Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....697ecacef49f393f4e437f067a29d2cf |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |