المؤلفون: Campillo Pedroza, Natalia, Franco Salazar, Juan Pablo, Gallego Gómez, Juan Carlos

المصدر: Revista Colombiana de Biotecnología; Vol. 17 Núm. 2 (2015); 123-129 ; Revista Colombiana de Biotecnología; Vol. 17 No. 2 (2015); 123-129 ; 1909-8758 ; 0123-3475

مصطلحات موضوعية: biología molecular, técnicas moleculares, investigación, 1-ethyl-3-(-3-dimethylaminopropyl) carboniimide), Digoxigenine, Northern blot non-radioactive, LNA probe, sRNAs

وصف الملف: application/vnd.openxmlformats-officedocument.wordprocessingml.document; application/pdf; text/html

العلاقة: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/48522/53583Test; https://revistas.unal.edu.co/index.php/biotecnologia/article/view/48522/53716Test; https://revistas.unal.edu.co/index.php/biotecnologia/article/view/48522/54118Test; Gomes, A. Q., Nolasco, S., & Soares, H. (2013). Non-coding RNAs: multi-tasking molecules in the cell. International Journal of Molecular Sciences, 14(8), 16010–39.; Kim, S. W., Li, Z., Moore, P. S., Monaghan, a P., Chang, Y., Nichols, M., & John, B. (2010). A sensitive non-radioactive northern blot method to detect small RNAs. Nucleic Acids Research, 38(7), e98.; Lee, R. C., Feinbaum, R. L., & Ambros, V. (1993). The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell, 75, 843–854. Pall, G. S., Codony-Servat, C., Byrne, J., Ritchie, L., & Hamilton, A. (2007). Carbodiimide-mediated cross-linking of RNA to nylon membranes improves the detection of siRNA, miRNA and piRNA by northern blot. Nucleic Acids Research, 35(8), e60.; Pall, G. S., & Hamilton, A. J. (2008). Improved northern blot method for enhanced detection of small RNA. Nature Protocols, 3, 1077–1084.; Petersen, M., Bondensgaard, K., Wengel, J., & Peter Jacobsen, J. (2002). Locked nucleic acid (LNA) recognition of RNA: NMR solution structures of LNA: RNA hybrids. Journal of the American Chemical Society, 124, 5974–5982.; Ramkissoon, S. H., Mainwaring, L. A., Sloand, E. M., Young, N. S., & Kajigaya, S. (2006). Nonisotopic detection of microRNA using digoxigenin labeled RNA probes. Molecular and Cellular Probes, 20, 1–4.; Sambrook, J., Fritsch, E. F., & Maniatis, T. (2001). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory (Vol. 3).; Van Wolfswinkel, J. C., & Ketting, R. F. (2010). The role of small non-coding RNAs in genome stability and chromatin organization. Journal of Cell Science, 123(Pt 11), 1825–39.; Várallyay, E., Burgyán, J., & Havelda, Z. (2008). MicroRNA detection by northern blotting using locked nucleic acid probes. Nature Protocols, 3(2), 190–6.; Wang, Z., & Yang, B. (2010). MicroRNA Expression detection methods. Heidelberg: Springer-Verlag.; Wengel, J. (1999). Synthesis of 3’-C-and 4'-C-Branched Oligodeoxynucleotides and the Development of Locked Nucleic Acid (LNA). Acc. Chem. Res., 32, 301–310.; Wengel, J. (2009). Locked Nucleic Acid Technology : A brief overview. Exiqon, 7.; Wightman, B., Ha, I., & Ruvkun, G. (1993). Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell, 75, 855–862.; Winter, J., Jung, S., Keller, S., Gregory, R. I., & Diederichs, S. (2009). Many roads to maturity: microRNA biogenesis pathways and their regulation. Nature Cell Biology, 11(3), 228–34.; https://revistas.unal.edu.co/index.php/biotecnologia/article/view/48522Test

الإتاحة: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/48522Test

المؤلفون: Campillo Pedroza, Natalia, Franco Salazar, Juan Pablo, Gallego Gómez, Juan Carlos

مصطلحات موضوعية: 6 Tecnología (ciencias aplicadas) / Technology, 66 Ingeniería química y Tecnologías relacionadas/ Chemical engineering, biología molecular, técnicas moleculares, investigación, 1-ethyl-3-(-3-dimethylaminopropyl) carboniimide), Digoxigenine, Northern blot non-radioactive, LNA probe, sRNAs

وصف الملف: application/pdf

العلاقة: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/48522Test; Universidad Nacional de Colombia Revistas electrónicas UN Revista Colombiana de Biotecnología; Revista Colombiana de Biotecnología; Campillo Pedroza, Natalia and Franco Salazar, Juan Pablo and Gallego Gómez, Juan Carlos (2015) Estandarización de un protocolo para Northern blot no radioactivo usado en la detección de pequeños RNA en células Vero. Revista Colombiana de Biotecnología, 17 (2). pp. 123-129. ISSN 1909-8758; https://repositorio.unal.edu.co/handle/unal/66727Test; http://bdigital.unal.edu.co/67755Test/

الإتاحة: https://repositorio.unal.edu.co/handle/unal/66727Test
http://bdigital.unal.edu.co/67755Test/

المؤلفون: Natalia Campillo Pedroza, Juan Pablo Franco Salazar, Juan Carlos Gallego Gómez

المصدر: Revista Colombiana de Biotecnología, Vol 17, Iss 2, Pp 123-129 (2015)

مصطلحات موضوعية: biología molecular, técnicas moleculares, investigación, 1-ethyl-3-(-3-dimethylaminopropyl) carboniimide), Digoxigenine, Northern blot non-radioactive, LNA probe, sRNAs, Biotechnology, TP248.13-248.65

العلاقة: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/48522Test; https://doaj.org/toc/0123-3475Test; https://doaj.org/toc/1909-8758Test; https://doaj.org/article/d6115d32be1d423096f1e01d5ae14331Test

الإتاحة: https://doi.org/10.15446/rev.colomb.biote.v17n2.48522Test
https://doaj.org/article/d6115d32be1d423096f1e01d5ae14331Test

المؤلفون: Jolivet Gougeon, Anne, Tamanai Shacoori, Zohreh, Pommepuy, Monique, Cormier, Michel

المصدر: Oceanologica Acta (0399-1784) (Elsevier), 2000-03 , Vol. 23 , N. 2 , P. 221-228

مصطلحات موضوعية: Bactéries viables non cultivables, Gène elt, Digoxigénine, PCR, Escherichia coli entérotoxinogènes (ETEC), Viable non culturable bacteria, Elt gene, Digoxigenin, Enterotoxigenic Escherichia coli ETEC

وصف الملف: application/pdf

العلاقة: https://archimer.ifremer.fr/doc/2000/publication-524.pdfTest; https://archimer.ifremer.fr/doc/00000/524Test/

الإتاحة: https://doi.org/10.1016/S0399-1784Test(00)00114-6
https://archimer.ifremer.fr/doc/2000/publication-524.pdfTest
https://archimer.ifremer.fr/doc/00000/524Test/

المؤلفون: Anne Jolivet-Gougeon, Michel Cormier, Monique Pommepuy, Zohreh Tamanai-Shacoori

المصدر: Oceanologica Acta (0399-1784) (Elsevier), 2000-03, Vol. 23, N. 2, P. 221-228

مصطلحات موضوعية: 0303 health sciences, 030306 microbiology, Chemistry, Environmental factor, Viable non culturable bacteria, Aquatic Science, Oceanography, medicine.disease_cause, Molecular biology, Gène elt, 03 medical and health sciences, PCR, Bactéries viables non cultivables, Escherichia coli entérotoxinogènes (ETEC), Digoxigénine, medicine, Elt gene, Enterotoxigenic Escherichia coli ETEC, Digoxigenin, 030304 developmental biology

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b487ef86c7af6bbf0de7d3cb4fdc0477Test
https://archimer.ifremer.fr/doc/00000/524Test/

المؤلفون: Vrieling, K., Peters, J., Sandbrink, J.M.

المصدر: Plant Molecular Biology Reporter 15 (1997) ; ISSN: 0735-9640

مصطلحات موضوعية: AFLP, Digoxigenine labelling, Non-radioactive labelling

العلاقة: https://research.wur.nl/en/publications/amplified-fragment-length-polymorphisms-aflps-detected-with-non-rTest

الإتاحة: https://research.wur.nl/en/publications/amplified-fragment-length-polymorphisms-aflps-detected-with-non-rTest

المؤلفون: Lu, Y.H., Merlino, M., Isaac, P.G., Staccy, J., Bernard, M., Leroy, P.

المساهمون: Revues Inra, Import

المصدر: Agronomie
Agronomie, EDP Sciences, 1994, 14 (1), pp.33-39
Agronomie 1 (14), 33-39. (1994)

مصطلحات موضوعية: [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, [SDV.SA] Life Sciences [q-bio]/Agricultural sciences, P32, ble tendre, rflp, food and beverages, sonde monocopie, Agricultural sciences, marquage non radioactif, [SDV.EE] Life Sciences [q-bio]/Ecology, environment, digoxigénine, triticum aestivum, Sciences agricoles, ComputingMilieux_MISCELLANEOUS

وصف الملف: application/pdf

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::f926cc78817dbfc12c08f2b4e9d12ddaTest
https://hal.archives-ouvertes.fr/hal-00885609/documentTest

المصدر: Oceanologica Acta (0399-1784) (Elsevier), 2000-03 , Vol. 23 , N. 2 , P. 221-228

مصطلحات الفهرس: Bactéries viables non cultivables, Gène elt, Digoxigénine, PCR, Escherichia coli entérotoxinogènes (ETEC), Viable non culturable bacteria, Elt gene, Digoxigenin, Enterotoxigenic Escherichia coli ETEC, text, Article, info:eu-repo/semantics/article

URL: https://archimer.ifremer.fr/doc/2000/publication-524.pdfTest
https://archimer.ifremer.fr/doc/00000/524Test/

المصدر: Oceanologica Acta (0399-1784) (Elsevier), 2000-03 , Vol. 23 , N. 2 , P. 221-228

مصطلحات الفهرس: Bactéries viables non cultivables, Gène elt, Digoxigénine, PCR, Escherichia coli entérotoxinogènes (ETEC), Viable non culturable bacteria, Elt gene, Digoxigenin, Enterotoxigenic Escherichia coli ETEC, text, Publication, info:eu-repo/semantics/article

المصدر: Oceanologica Acta (0399-1784) (Elsevier), 2000-03 , Vol. 23 , N. 2 , P. 221-228

مصطلحات الفهرس: Viable non culturable bacteria, Elt gene, Digoxigenin, PCR, Enterotoxigenic Escherichia coli ETEC, Bactéries viables non cultivables, Gène elt, Digoxigénine, Escherichia coli entérotoxinogènes (ETEC), text, Publication

URL: http://archimer.ifremer.fr/doc/2000/publication-524.pdfTest
http://archimer.ifremer.fr/doc/00000/524Test/
http://archimer.ifremer.fr/doc/00000/524Test