Pretreatment of Body Fluid Specimens Using Hyaluronidase and Ultracentrifugation
| العنوان: | Pretreatment of Body Fluid Specimens Using Hyaluronidase and Ultracentrifugation |
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| المؤلفون: | Emily Zupan, Devon R Turner, Jonathan R. Genzen, Sonia L. La'ulu |
| المصدر: | Laboratory Medicine. 52:469-476 |
| بيانات النشر: | Oxford University Press (OUP), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Body fluid, Creatinine, Chromatography, biology, Bilirubin, Sodium, Biochemistry (medical), Clinical Biochemistry, Hyaluronoglucosaminidase, Body Fluids, chemistry.chemical_compound, Carcinoembryonic antigen, chemistry, Hyaluronidase, Lactate dehydrogenase, biology.protein, medicine, Humans, Uric acid, Amylase, Ultracentrifugation, Laboratories, Clinical, medicine.drug |
| الوصف: | Objective Viscous body fluids present challenges during clinical laboratory testing. The present study was conducted to evaluate the effectiveness of hyaluronidase (HYAL) and ultracentrifugation (UC) pretreatment for a variety of body fluids before clinical chemistry testing. Methods The following body fluids were evaluated: biliary/hepatic, cerebrospinal, dialysate, drain, pancreatic, pericardial, peritoneal/ascites, pleural, synovial, and vitreous. Analytes assessed included amylase, total bilirubin, cancer antigen 19-9, carcinoembryonic antigen, cholesterol, chloride, creatinine, glucose, lactate dehydrogenase, lipase, potassium, rheumatoid factor, sodium, total protein, triglycerides, urea nitrogen, and uric acid. Results Observed percentage differences between HYAL treated and untreated fluids were less than ±15% for all analytes investigated, with a small number showing statistical significance (P Conclusion The HYAL treatment effectively reduced viscosity for body fluids. Validation of specimen pretreatment processes ensures acceptable analytical performance and the absence of unanticipated interferences. |
| تدمد: | 1943-7730 0007-5027 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15cef1184cf884ab9f49f7c797709d15Test https://doi.org/10.1093/labmed/lmaa115Test |
| حقوق: | CLOSED |
| رقم الانضمام: | edsair.doi.dedup.....15cef1184cf884ab9f49f7c797709d15 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19437730 00075027 |
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