دورية أكاديمية
PUB-NChIP--'in vivo biotinylation' approach to study chromatin in proximity to a protein of interest
| العنوان: | PUB-NChIP--'in vivo biotinylation' approach to study chromatin in proximity to a protein of interest |
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| المؤلفون: | Shoaib, Muhammad, Kulyyassov, Arman, Robin, Chloé, Winczura, Kinga, Tarlykov, Pavel, Despas, Emmanuelle, Kannouche, Patricia, Ramanculov, Erlan, Lipinski, Marc, Ogryzko, Vasily |
| بيانات النشر: | Cold Spring Harbor Laboratory Press |
| سنة النشر: | 2013 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | METHOD |
| الوصف: | We have developed an approach termed PUB-NChIP (proximity utilizing biotinylation with native ChIP) to purify and study the protein composition of chromatin in proximity to a nuclear protein of interest. It is based on coexpression of (1) a protein of interest, fused with the bacterial biotin ligase BirA, together with (2) a histone fused to a biotin acceptor peptide (BAP), which is specifically biotinylated by BirA-fusion in the proximity of the protein of interest. Using the RAD18 protein as a model, we demonstrate that the RAD18-proximal chromatin is enriched in some H4 acetylated species. Moreover, the RAD18-proximal chromatin containing a replacement histone H2AZ has a different pattern of H4 acetylation. Finally, biotin pulse-chase experiments show that the H4 acetylation pattern starts to resemble the acetylation pattern of total H4 after the proximity of chromatin to RAD18 has been lost. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://genome.cshlp.org/cgi/content/short/23/2/331Test; http://dx.doi.org/10.1101/gr.134874.111Test |
| DOI: | 10.1101/gr.134874.111 |
| الإتاحة: | https://doi.org/10.1101/gr.134874.111Test http://genome.cshlp.org/cgi/content/short/23/2/331Test |
| حقوق: | Copyright (C) 2013, Cold Spring Harbor Laboratory Press |
| رقم الانضمام: | edsbas.731B4036 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1101/gr.134874.111 |
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