Neurine, an acetylcholine autolysis product, elevates secreted amyloid-β protein precursor and amyloid-β peptide levels, and lowers neuronal cell viability in culture: A role in Alzheimer's disease?
| العنوان: | Neurine, an acetylcholine autolysis product, elevates secreted amyloid-β protein precursor and amyloid-β peptide levels, and lowers neuronal cell viability in culture: A role in Alzheimer's disease? |
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| المؤلفون: | De Moa Chen, Nigel H. Greig, Jason A. Bailey, Arnold Brossi, David Tweedie, Qian Sheng Yu, Debomoy K. Lahiri, Kumar Sambamurti, Mohammad Amjad Kamal, Yuan Wen Ge |
| المصدر: | Journal of Alzheimer's Disease. 10:9-16 |
| بيانات النشر: | IOS Press, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | medicine.medical_specialty, Cell Survival, Amyloid beta, Blotting, Western, Cell Culture Techniques, Amyloid beta-Protein Precursor, Neuroblastoma, Alzheimer Disease, Internal medicine, Tumor Cells, Cultured, medicine, Humans, Viability assay, Cholinergic neuron, Protein precursor, Cell Proliferation, Neurons, Amyloid beta-Peptides, Cell Death, L-Lactate Dehydrogenase, biology, Cell growth, General Neuroscience, Cell Adhesion Molecules, Neuron-Glia, General Medicine, Molecular biology, Acetylcholine, Psychiatry and Mental health, Clinical Psychology, Endocrinology, Cell culture, biology.protein, Cholinergic, Geriatrics and Gerontology, Autolysis, medicine.drug |
| الوصف: | Classical hallmarks of Alzheimer's disease (AD) are a synaptic loss, cholinergic neuron death, and abnormal protein deposition, particularly of toxic amyloid-beta peptide (Abeta) that is derived from amyloid-beta protein precursor (AbetaPP) by the action of beta- and gamma-secretases. The trigger(s) initiating the biochemical cascades that underpin these hallmarks have yet to be fully elucidated. The typical forebrain cholinergic cell demise associated with AD brain results in a loss of presynaptic cholinergic markers and acetylcholine (ACh). Neurine (vinyl-trimethyl-ammonium hydroxide) is a breakdown product of ACh, consequent to autolysis and is an organic poison found in cadavre brain. The time- and concentration-dependent actions of neurine were assessed in human neuroblastoma (NB, SK-N-SH) cells in culture by quantifying cell viability by lactate dehydrogenase (LDH) and MTS assay, and AbetaPP and Abeta levels by Western blot and ELISA. NB cells displayed evidence of toxicity to neurine at > or = 3 mg/ml, as demonstrated by elevated LDH levels in the culture media and a reduced cell viability shown by the MTS assay. Using subtoxic concentrations of neurine, elevations in AbetaPP and Abeta1-40 peptide levels were detected in conditioned media samples. |
| تدمد: | 1875-8908 1387-2877 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ca36b86d1a7f3f00fd405f0a906f3218Test https://doi.org/10.3233/jad-2006-10102Test |
| رقم الانضمام: | edsair.doi.dedup.....ca36b86d1a7f3f00fd405f0a906f3218 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 18758908 13872877 |
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