التفاصيل البيبلوغرافية
| العنوان: |
Using Nested PCR To Detect the hlyA Gene of Listeria monocytogenes in Minas Frescal Cow's Milk Cheese. |
| المؤلفون: |
DE OLIVEIRA SOUZA, STEFANIA MÁRCIA, SARMENTO BRASILEIRO, ISABELA, FERNANDES DE CARVALHO, ANTONIO, DA SILVA JR., ABELARDO, AUGUSTO NERO, LUÍS, DE AGUIAR FERREIRA, MÁRCIA |
| المصدر: |
Journal of Food Protection; Jul2012, Vol. 75 Issue 7, p1324-1327, 4p |
| مصطلحات موضوعية: |
LISTERIA monocytogenes, LISTERIOSIS in animals, CHEESE analysis, FOOD pathogens, POLYMERASE chain reaction |
| مستخلص: |
To evaluate a nested PCR protocol for Listeria monocytogenes detection, Minas Frescal cow's milk cheeses were produced, artificially inoculated with this pathogen at concentrations ranging from 1 to 1,000 CFU/g, and stored at 4°C for 10 days. The International Organization for Standardization (tSO) standardized method 11290-1/A1 was used to detect L. monocytogenes in the inoculated samples, and DNA was extracted from aliquots (1, 5, and l0 ml) of 1:10 dilution, followed by a nested PCR protocol for the hlyA gene. The ISO standardized reference method and nested PCR both detected L. monocytogenes at all concentrations and during all storage periods; McNemar's test showed no significant difference (P > 0.05). The results indicated that the nested PCR protocol can be used as a screening test to detect L. monocytogenes in Minas Frescal cheese, allowing earlier detection of the pathogen that can later be confirmed by the ISO standardized reference method. [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
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