دورية أكاديمية
Defective bone repair in mast cell deficient mice with c-Kit loss of function
| العنوان: | Defective bone repair in mast cell deficient mice with c-Kit loss of function |
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| المؤلفون: | DA Behrends, L Cheng, MB Sullivan, MH Wang, GB Roby, N Zayed, C Gao, JE Henderson, PA Martineau |
| المصدر: | European Cells & Materials, Vol 28, Pp 209-222 (2014) |
| بيانات النشر: | Forum Multimedia Publishing LLC, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Diseases of the musculoskeletal system LCC:Orthopedic surgery |
| مصطلحات موضوعية: | Osteo-immunology, c-Kit and mast cell deficiency, re-vascularisation, bone regeneration, Diseases of the musculoskeletal system, RC925-935, Orthopedic surgery, RD701-811 |
| الوصف: | KitW-sh mice carry an inactivating mutation in the gene encoding the receptor for stem cell factor, which is expressed at high levels on the surface of haematopoietic precursor cells. The mutation results in mast cell deficiency, a variety of defects in innate immunity and poorly defined abnormalities in bone. The present study was designed to characterise healing of a cortical window defect in skeletally mature KitW-sh mice using high-resolution micro computed tomographic imaging and histological analyses. The cortical bone defect healed completely in all wild type mice but failed to heal in about half of the KitW-sh mice by 12 weeks post-operative. Defective healing was associated with premature and excessive expression of TRAP positive cells embedded in fibrous marrow but with little change in ALP activity. Immuno-histochemical analyses revealed reduced CD34 positive vascular endothelial cells and F4/80 positive macrophages at 1 and 2 weeks post-operative. Impaired bone healing in the KitW-sh mice was therefore attributed to altered catabolic activity, impaired re-vascularisation and compromised replacement of woven with compact bone. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1473-2262 |
| العلاقة: | http://www.ecmjournal.org/journal/papers/vol028/pdf/v028a14.pdfTest; https://doaj.org/toc/1473-2262Test |
| الوصول الحر: | https://doaj.org/article/e5ad815ae0d945618ff979ecfa34a269Test |
| رقم الانضمام: | edsdoj.5ad815ae0d945618ff979ecfa34a269 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14732262 |
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