التفاصيل البيبلوغرافية
| العنوان: |
The development of lateral flow devices for urinary biomarkers to assess kidney health. |
| المؤلفون: |
D Souza, Serena1 (AUTHOR), Obeid, Wassim1 (AUTHOR), Hernandez, Jeanine1 (AUTHOR), Hu, David1 (AUTHOR), Wen, Yumeng1 (AUTHOR), Moledina, Dennis G.2 (AUTHOR), Albert, Andre3 (AUTHOR), Gregg, Anya4 (AUTHOR), Wheeler, Andrew3 (AUTHOR), Philbrook, Heather Thiessen1 (AUTHOR), Parikh, Chirag R.1 (AUTHOR) chirag.parikh@jhmi.edu |
| المصدر: |
Scientific Reports. 4/12/2024, Vol. 14 Issue 1, p1-8. 8p. |
| مصطلحات موضوعية: |
*BIOMARKERS, *KIDNEY physiology, *KIDNEYS, *INTERLEUKIN-9, *UROMODULIN |
| مستخلص: |
Serum creatinine levels are insensitive to real-time changes in kidney function or injury. There is a growing interest in assessing kidney injury by measuring biomarkers in body fluid. From our previous studies, we identified and reported three urinary biomarkers namely Uromodulin (UMOD), Osteopontin (OPN), and Interleukin-9 (IL-9) to be associated with kidney health. The availability of a rapid point-of-care test for these urinary biomarkers will potentially accelerate its applicability and accessibility. In this study, we aimed to develop novel lateral flow device (LFD) for UMOD, OPN and IL-9. We tested paired antibodies using Enzyme Linked Immunosorbent Assay wherein we observed functionality only for UMOD and OPN and not for IL-9. A conjugation buffer pH of 7.8 and 8.5 was found suitable at a detection antibody concentration of 15 µg/mL for LFD development. The developed LFDs were found to quantitatively measure UMOD standard (LLOD of 80,000 pg/mL) and OPN standard (LLOD of 8600 pg/mL) respectively. The LFD was also able to measure human urinary UMOD and OPN with a percent CV of 12.12 and 5.23 respectively. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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