دورية أكاديمية
MT1-MMP-Mediated cleavage of decorin in corneal angiogenesis
| العنوان: | MT1-MMP-Mediated cleavage of decorin in corneal angiogenesis |
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| المؤلفون: | Kojima, T, Onguchi, T, Han, KY, Zhou, Z, Azar, DT, Chang, JH, Mimura, T, Kim, TI |
| بيانات النشر: | //www.karger.com/JVR Switzerland |
| سنة النشر: | 2009 |
| المجموعة: | University of Hong Kong: HKU Scholars Hub |
| مصطلحات موضوعية: | Metalloproteinase, Membrane type 1-matrix metalloproteinase, Extracellular matrix, Decorin, Corneal neovascularization, Angiogenesis, MMP-14, Proteoglycans - metabolism, Cornea - enzymology, Corneal Neovascularization - chemically induced - enzymology, Extracellular Matrix Proteins - metabolism, Matrix Metalloproteinase 14 - antagonists and inhibitors - deficiency - genetics - metabolism |
| الوصف: | Background/Aims: Decorin has been shown to have antiangiogenic properties. In this study, we evaluate the involvement of membrane type 1-matrix metalloproteinase (MT1-MMP), a proangiogenic enzyme, in decorin cleavage in the cornea. Methods: MT1-MMP expression was confirmed immunohistochemically in keratocytes and immortalized corneal fibroblast cell lines. Corneal micropockets of bFGF were used to assess the expression of decorin and MT1-MMP. Western blotting was used to evaluate decorin degradation by MT1-MMP. Aortic ring tube formation assays were used to assay the inhibitory effect of decorin and stimulatory effect of MT1-MMP on vascular endothelial cells in vitro. Results: We show that MT1-MMP expression is upregulated following bFGF pellet implantation in the cornea in vivo, and that MT1-MMP cleaves decorin in a time-and concentration-dependent manner in vitro. Furthermore, the addition of MT1-MMP reduces the inhibitory effects of decorin on aortic ring tube formation in vitro. Cleavage of decorin by MT1-MMP-deficient corneal cell lysates is diminished relative to that by wild-type corneal cell lysates, and an MT1-MMP knockin restores decorin processing in vitro. Conclusion: The proangiogenic role of MT1-MMP in the cornea may be mediated, in part, by facilitated cleavage of corneal decorin. © 2009 S. Karger AG, Basel. ; link_to_subscribed_fulltext |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-00-270780-0 0-00-270780-2 |
| تدمد: | 1018-1172 |
| العلاقة: | Journal of Vascular Research; http://www.scopus.com/mlt/select.url?eid=2-s2.0-67649424445&selection=ref&src=s&origin=recordpageTest; Journal Of Vascular Research, 2009, v. 46 n. 6, p. 541-550; 550; 167558; WOS:000270780200003; http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1018-1172&volume=46&issue=6&spage=541&epage=550&date=2009&atitle=MT1-MMP-mediated+cleavage+of+decorin+in+corneal+angiogenesisTest; eid_2-s2.0-67649424445; 541; http://hdl.handle.net/10722/58308Test; 46 |
| DOI: | 10.1159/000226222 |
| الإتاحة: | https://doi.org/10.1159/000226222Test http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1018-1172&volume=46&issue=6&spage=541&epage=550&date=2009&atitle=MT1-MMP-mediated+cleavage+of+decorin+in+corneal+angiogenesisTest http://hdl.handle.net/10722/58308Test |
| حقوق: | Journal of Vascular Research. Copyright © S Karger AG. |
| رقم الانضمام: | edsbas.5281737F |
| قاعدة البيانات: | BASE |
| ردمك: | 9780002707800 0002707802 |
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| تدمد: | 10181172 |
| DOI: | 10.1159/000226222 |