التفاصيل البيبلوغرافية
| العنوان: |
Direct Air Capture of CO 2 by Microalgae with Buoyant Beads Encapsulating Carbonic Anhydrase |
| المؤلفون: |
Xiaoyin Xu (773234), Sandra E. Kentish (623788), Gregory J. O. Martin (8450952) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Neuroscience, Physiology, Pharmacology, Biotechnology, Ecology, Plant Biology, Space Science, Environmental Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, Direct Air Capture, GA, paraffin-embedded alginate framework, calcium alginate beads, 10 assay cycles, Buoyant Beads Encapsulating Carboni., CO 2, microalgae cultivation, CA, mg, Coomassie Blue dyeing |
| الوصف: |
Microalgae cultures have promise as a CO 2 sink for atmospheric carbon and as a sustainable source of food and chemical feedstocks. However, large-scale microalgae cultivation is currently limited by the need to provide carbon dioxide from point sources, as the diffusion of atmospheric CO 2 is too slow. Carbonic anhydrase (CA) is an effective enzyme to facilitate the dissolution of atmospheric CO 2 that could be used to enhance the photosynthetic uptake of this greenhouse gas. Here we investigate a means of retaining CA at the surface of algae ponds to facilitate direct air capture by cross-linking CA with glutaraldehyde (GA) before encapsulation into buoyant calcium alginate beads. Coomassie Blue dyeing and Wilbur–Anderson assays confirmed the successful bonding of CA to the beads. Microscopic images showed the paraffin-embedded alginate framework. The CA–GA beads retain virtually all hydrase activity throughout 10 assay cycles. Compared with a natural growth rate of 22.7 ± 0.5 mg L –1 day –1 , free CA and CA–GA beads increased the productivity of Nannochloropsis salina to 37 ± 3 mg L –1 day –1 and 40 ± 1 mg L –1 day –1 , respectively. The CA–GA beads further provided a stable growth enhancement for three rounds of microalgae cultivation, confirming that these buoyant beads can be readily recovered and re-used, which is promising for industrial biomass production. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/Direct_Air_Capture_of_CO_sub_2_sub_by_Microalgae_with_Buoyant_Beads_Encapsulating_Carbonic_Anhydrase/14988683Test |
| DOI: |
10.1021/acssuschemeng.1c01618.s001 |
| الإتاحة: |
https://doi.org/10.1021/acssuschemeng.1c01618.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.DFC933B3 |
| قاعدة البيانات: |
BASE |
