دورية أكاديمية
Improved Diagnosis of Viable Parenchymal Neurocysticercosis by Combining Antibody Banding Patterns on Enzyme-Linked Immunoelectrotransfer Blot (EITB) with Antigen Enzyme-Linked Immunosorbent Assay (ELISA)
العنوان: | Improved Diagnosis of Viable Parenchymal Neurocysticercosis by Combining Antibody Banding Patterns on Enzyme-Linked Immunoelectrotransfer Blot (EITB) with Antigen Enzyme-Linked Immunosorbent Assay (ELISA) |
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المؤلفون: | Arroyo, G., Bustos, J.A., Lescano, A.G., Gonzales, I., Saavedra, H., Pretell, E.J., Castillo, Y., Perez, E., Dorny, P., Gilman, R.H., O'Neal, S.E., Gonzalez, A.E., Garcia, H.H., Cysticercosis Working Group in Peru (CWGP) |
بيانات النشر: | American Society for Microbiology |
سنة النشر: | 2022 |
المجموعة: | Repositorio - UPCH (Universidad Peruana Cayetano Heredia) |
مصطلحات موضوعية: | Parenchymal Neurocysticercosis, Combining Antibody Banding Patterns, Enzyme-Linked Immunoelectrotransfer Blot, Antigen Enzyme-Linked Immunosorbent Assay |
الوصف: | The diagnosis of neurocysticercosis (NCC) depends on neuroimaging and serological confirmation. While antibody detection by enzyme-linked immunoelectrotransfer blot (EITB) fails to predict viable NCC, EITB banding patterns provide information about the host's infection course. Adding antigen enzyme-linked immunosorbent assay (Ag-ELISA) results to EITB banding patterns may improve their ability to predict or rule out of viable NCC. We assessed whether combining EITB banding patterns with Ag-ELISA improves discrimination of viable infection in imaging-confirmed parenchymal NCC. EITB banding patterns were grouped into classes using latent class analysis. True-positive and false-negative Ag-ELISA results in each class were compared using Fisher's exact test. Four classes were identified: 1, EITB negative or positive to GP50 alone (GP50 antigen family); 2, positive to GP42-39 and GP24 (T24/42 family), with or without GP50; and 3 and 4, positive to GP50, GP42-39, and GP24 and reacting to bands in the 8-kDa family. Most cases in classes 3 and 4 had viable NCC (82% and 88%, respectively) compared to classes 2 and 1 (53% and 5%, respectively). Adding positive Ag-ELISA results to class 2 predicted all viable NCC cases (22/22 [100%]), whereas 11/40 patients (27.5%) Ag-ELISA negative had viable NCC (P < 0.001). Only 1/4 patients (25%) Ag-ELISA positive in class 1 had viable NCC, whereas 1/36 patients (2.8%) Ag-ELISA negative had viable NCC (P = 0.192). In classes 3 and 4, adding Ag-ELISA was not contributory. Combining Ag-ELISA with EITB banding patterns improves discrimination of viable from nonviable NCC, particularly for class 2 responses. Together, these complement neuroimaging more appropriately for the diagnosis of viable NCC. |
نوع الوثيقة: | article in journal/newspaper |
اللغة: | English |
تدمد: | 1098-660X |
العلاقة: | urn:issn:1098-660X; https://hdl.handle.net/20.500.12866/11752Test; Journal of Clinical Microbiology; https://doi.org/10.1128/JCM.01550-21Test |
DOI: | 10.1128/JCM.01550-21 |
الإتاحة: | https://doi.org/20.500.12866/11752Test https://doi.org/10.1128/JCM.01550-21Test https://hdl.handle.net/20.500.12866/11752Test |
حقوق: | info:eu-repo/semantics/restrictedAccess ; https://creativecommons.org/licenses/by-nc-nd/4.0/deed.esTest |
رقم الانضمام: | edsbas.E7788B75 |
قاعدة البيانات: | BASE |
تدمد: | 1098660X |
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DOI: | 10.1128/JCM.01550-21 |