Legionella pneumophila Effector RomA Uniquely Modifies Host Chromatin to Repress Gene Expression and Promote Intracellular Bacterial Replication
| العنوان: | Legionella pneumophila Effector RomA Uniquely Modifies Host Chromatin to Repress Gene Expression and Promote Intracellular Bacterial Replication |
|---|---|
| المؤلفون: | Tobias Sahr, Clement Bertholet, Carmen Buchrieser, Christophe Rusniok, Raphaël Margueron, Serena Sanulli, Monica Rolando, Laura Gomez-Valero |
| المساهمون: | Biologie des Bactéries intracellulaires - Biology of Intracellular Bacteria, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Génétique et Biologie du Développement, Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Work in the C. Buchrieser laboratory is supported by the Institut Carnot-Pasteur MI, the Fondation pour la Recherche Médicale (FRM), the French Region Ile de France (DIM Malinf), and grant ANR-10-LABX-62-IBEID. M.R. was supported by the ANR-08-PATH-002-02 project FunGen (ERA-Net PathoGenoMics) and a Roux contract financed by the Institut Pasteur., Work in the R.M. laboratory is supported by the Institut National du Cancer, the FRM, and the European Research Council (ERC-stg). S.S. is supported by a PhD extension from the Association pour la Recherche sur le Cancer (ARC)., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-08-PATH-0002,FUNGEN(2008), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut Curie [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Cell Host and Microbe Cell Host and Microbe, Elsevier, 2013, 13 (4), pp.395-405. ⟨10.1016/j.chom.2013.03.004⟩ Cell Host and Microbe, 2013, 13 (4), pp.395-405. ⟨10.1016/j.chom.2013.03.004⟩ |
| بيانات النشر: | Elsevier BV, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Cancer Research, MESH: Chromatin/immunology, MESH: Amino Acid Sequence, MESH: Promoter Regions, Genetic/immunology, Legionella pneumophila, MESH: Gene Expression/immunology, MESH: Immunity, Innate/immunology, MESH: Bacterial Proteins/immunology, MESH: Histones/immunology, Host cell nucleus, Genetics, 0303 health sciences, biology, Effector, MESH: Transcription, Genetic/genetics, MESH: DNA Replication/immunology, Chromatin, Cell biology, MESH: Nuclear Proteins/immunology, Histone, MESH: Transcription, Genetic/immunology, Histone methyltransferase, MESH: Chromatin/genetics, MESH: Bacterial Proteins/genetics, MESH: Nuclear Proteins/genetics, MESH: Immunity, Innate/genetics, MESH: Sequence Alignment, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Microbiology, MESH: Legionella pneumophila/genetics, MESH: Methylation, 03 medical and health sciences, Histone H3, MESH: Histones/genetics, Virology, Immunology and Microbiology(all), MESH: Gene Expression/genetics, Epigenetics, Molecular Biology, 030304 developmental biology, MESH: Molecular Sequence Data, MESH: Humans, 030306 microbiology, biology.organism_classification, MESH: Cell Line, MESH: DNA Replication/genetics, MESH: Legionella pneumophila/immunology, MESH: Promoter Regions, Genetic/genetics, biology.protein, Parasitology |
| الوصف: | International audience; Histone posttranslational modifications control eukaryotic gene expression and regulate many biological processes including immunity. Pathogens alter host epigenetic control to aid pathogenesis. We find that the intracellular bacterial pathogen Legionella pneumophila uses a Dot/Icm type IV secreted effector, RomA, to uniquely modify the host chromatin landscape. RomA, a SET domain-containing methyltransferase, trimethylates K14 of histone H3, a histone mark not previously described in mammals. RomA localizes to the infected cell nucleus where it promotes a burst of H3K14 methylation and consequently decreases H3K14 acetylation, an activating histone mark, to repress host gene expression. ChIP-seq analysis identified 4,870 H3K14 methylated promoter regions, including innate immune genes. Significantly reduced replication of a RomA-deleted strain in host cells was trans-complemented by wild-type, but not by catalytically inactive, RomA. Thus, a secreted L. pneumophila effector targets the host cell nucleus and modifies histones to repress gene expression and promote efficient intracellular replication. Copyright © 2013 Elsevier Inc. All rights reserved. |
| تدمد: | 1931-3128 |
| DOI: | 10.1016/j.chom.2013.03.004 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff7f9ba679975f15521adf796cc088f3Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ff7f9ba679975f15521adf796cc088f3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19313128 |
|---|---|
| DOI: | 10.1016/j.chom.2013.03.004 |