دورية أكاديمية
Significance of sphingosine kinase 1 expression in feline mammary tumors
| العنوان: | Significance of sphingosine kinase 1 expression in feline mammary tumors |
|---|---|
| المؤلفون: | 張益銍, Chang, Yi-Chih, 莊曉莉, Chuang3, Hsiao-Li, Yin, Ji-Hang, Jiunn-Wang, L, Liao, Jiunn-Wang, 陳德勛, Chen, Ter-Hsin, 王裕智, Wang, Yu-Chih |
| المساهمون: | 生物科技學系 |
| سنة النشر: | 2019 |
| المجموعة: | ASIA University, Taiwan: Instiutional Repository (ASIAIR) / 歡迎光臨亞洲大學全球資訊網 |
| الوصف: | Background Sphingosine kinase 1 (SPHK1) is an enzyme that converts pro-apoptotic ceramide and sphingosine into anti-apoptotic sphingosine-1-phosphate. There is growing evidence that SPHK1 activation promotes oncogenic transformation, tumor growth, chemotherapy resistance, and metastatic spread. High SPHK1 expression has been associated with a poor prognosis in several human cancers. Results In the present study, the expression level of SPHK1 was examined in feline mammary tumor (FMT) specimens, and the IHC expression level of SPHK1 was associated with the histological grade of FMTs. IHC analysis of 88 FMT cases revealed that the expression level of SPHK1 was upregulated in 53 tumor tissues (60.2%) compared to adjacent mammary tissues. SPHK1 expression in FMTs was significantly associated with histological grade, presence of lymphovascular invasion, and estrogen receptor negativity. Treatment of primary FMT cells with SPHK1 inhibitors reduced cell viability, indicating that SPHK1 acts to promote FMT cell survival. These results indicate that SPHK1 may play an important role in FMTs and may be a therapeutic target in cats with FMT. Conclusions SPHK1 over-expression in breast cancer tissues is associated with a poor prognosis in humans. SPHK1 over-expression in more aggressive FMTs provides support for a potential role of SPHK1 inhibitors for the treatment of FMTs. Targeting SPHK1 has potent cytotoxic effects in primary FMT cells. These findings suggest that further examination of the role SPHK1 plays in FMTs will pave the way for the investigation of SPHK1 inhibitors in future clinical applications. Electronic supplementary material The online version of this article (10.1186/s12917-019-1883-z) contains supplementary material, which is available to authorized users. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | Chinese |
| العلاقة: | BMC Veterinary Research; http://asiair.asia.edu.tw/ir/handle/310904400/112429Test; http://asiair.asia.edu.tw/ir/bitstream/310904400/112429/2/index.htmlTest |
| الإتاحة: | http://asiair.asia.edu.tw/ir/handle/310904400/112429Test http://asiair.asia.edu.tw/ir/bitstream/310904400/112429/2/index.htmlTest |
| رقم الانضمام: | edsbas.893629D7 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |