دورية أكاديمية
A rapid point-of-care assay accurately measures vitamin D
| العنوان: | A rapid point-of-care assay accurately measures vitamin D |
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| المؤلفون: | Albrecht, K., Lotz, J., Frommer, L., Lackner, K. J., Kahaly, George J. |
| المصدر: | http://lobid.org/resources/99370679184906441Test#!, 44(11):2485-2492. |
| سنة النشر: | 2021 |
| المجموعة: | Publisso (ZB MED-Publikationsportal Lebenswissenschaften) |
| مصطلحات موضوعية: | Point-of-Care Systems [MeSH], Vitamin D Deficiency/blood [MeSH], Chemiluminescence assay, Humans [MeSH], Vitamin D serum levels, Cholecalciferol/blood [MeSH], Dimensional Measurement Accuracy [MeSH], Point-of-care, Luminescent Measurements/methods [MeSH], Vitamin D Deficiency/diagnosis [MeSH], Rapid On-site Evaluation [MeSH], Original Article, Immunofluorescence assay, Reproducibility of Results [MeSH], Cholecalciferol/analysis [MeSH], Fluorescent Antibody Technique/methods [MeSH], Rapid assay |
| الوصف: | Purpose!#!Vitamin D (VitD) is a pleiotropic hormone with effects on a multitude of systems and metabolic pathways. Consequently, the relevance of a sufficiently high VitD serum level becomes self-evident.!##!Methods!#!A rapid immunofluorescence assay designed for the point-of-care measurement of serum VitD!##!Results!#!An overall linear correlation of r = 0.89 (Pearson, 95% CI 0.88-0.92, p < 0.01) between the point of care and the conventional reference assay was registered. Accuracy and precision were of special interest at cut-points (10 ng/ml [mean deviation 1.7 ng/ml, SD 1.98 ng/ml, SE 0.16 ng/ml], 12 ng/ml [MD 0.41, SD 1.89, SE 0.19] and 30 ng/ml [MD - 1.11, SD 3.89, SE 0.35]). Only a slight deviation was detected between the two assays when using fresh (r = 0.91, 95% CI 0.86-0.94, p < 0.01) and frozen serum samples (r = 0.86, 0.82-0.89, p < 0.01). Results remained steady when samples were frozen several times. Inter- and intra-assay validation according to the CLSI protocol as well as multiuser testing showed stable results.!##!Conclusion!#!This novel, innovative, and controlled study indicates that the evaluated rapid point of care VitD assay is reliable, accurate, and suited for clinical practice. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://repository.publisso.de/resource/frl:6445352Test; https://doi.org/10.1007/s40618-021-01575-8Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502163Test/ |
| DOI: | 10.1007/s40618-021-01575-8 |
| الإتاحة: | https://doi.org/10.1007/s40618-021-01575-8Test https://repository.publisso.de/resource/frl:6445352Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8502163Test/ |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.E8A09823 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s40618-021-01575-8 |
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