دورية أكاديمية
Investigation of the utility of the 1.1B4 cell as a model human beta cell line for study of persistent enteroviral infection
| العنوان: | Investigation of the utility of the 1.1B4 cell as a model human beta cell line for study of persistent enteroviral infection |
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| المؤلفون: | Chaffey, Jessica R., Young, Jay, Leslie, Kaiyven A., Partridge, Katie, Akhbari, Pouria, Dhayal, Shalinee, Hill, Jessica L., Wedgwood, Kyle C. A., Burnett, Edward, Russell, Mark A., Richardson, Sarah J., Morgan, Noel G. |
| المساهمون: | Diabetes UK, Juvenile Diabetes Research Foundation International, Medical Research Council |
| المصدر: | Scientific Reports ; volume 11, issue 1 ; ISSN 2045-2322 |
| بيانات النشر: | Springer Science and Business Media LLC |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Multidisciplinary |
| الوصف: | The generation of a human pancreatic beta cell line which reproduces the responses seen in primary beta cells, but is amenable to propagation in culture, has long been an important goal in diabetes research. This is particularly true for studies focussing on the role of enteroviral infection as a potential cause of beta-cell autoimmunity in type 1 diabetes. In the present work we made use of a clonal beta cell line (1.1B4) available from the European Collection of Authenticated Cell Cultures, which had been generated by the fusion of primary human beta-cells with a pancreatic ductal carcinoma cell, PANC-1. Our goal was to study the factors allowing the development and persistence of a chronic enteroviral infection in human beta-cells. Since PANC-1 cells have been reported to support persistent enteroviral infection, the hybrid 1.1B4 cells appeared to offer an ideal vehicle for our studies. In support of this, infection of the cells with a Coxsackie virus isolated originally from the pancreas of a child with type 1 diabetes, CVB4.E2, at a low multiplicity of infection, resulted in the development of a state of persistent infection. Investigation of the molecular mechanisms suggested that this response was facilitated by a number of unexpected outcomes including an apparent failure of the cells to up-regulate certain anti-viral response gene products in response to interferons. However, more detailed exploration revealed that this lack of response was restricted to molecular targets that were either activated by, or detected with, human-selective reagents. By contrast, and to our surprise, the cells were much more responsive to rodent-selective reagents. Using multiple approaches, we then established that populations of 1.1B4 cells are not homogeneous but that they contain a mixture of rodent and human cells. This was true both of our own cell stocks and those held by the European Collection of Authenticated Cell Cultures. In view of this unexpected finding, we developed a strategy to harvest, isolate and ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1038/s41598-021-94878-y |
| الإتاحة: | https://doi.org/10.1038/s41598-021-94878-yTest https://www.nature.com/articles/s41598-021-94878-y.pdfTest https://www.nature.com/articles/s41598-021-94878-yTest |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test ; https://creativecommons.org/licenses/by/4.0Test |
| رقم الانضمام: | edsbas.CAD82C4D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-021-94878-y |
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