دورية أكاديمية
Study on pyroptosis-related genes Casp8, Gsdmd and Trem2 in mice with cerebral infarction
| العنوان: | Study on pyroptosis-related genes Casp8, Gsdmd and Trem2 in mice with cerebral infarction |
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| المؤلفون: | Shunli Liang, Linsheng Xu, Xilin Xin, Rongbo Zhang, You Wu |
| المصدر: | PeerJ, Vol 12, p e16818 (2024) |
| بيانات النشر: | PeerJ Inc., 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine LCC:Biology (General) |
| مصطلحات موضوعية: | Cerebral infarction, Bioinformatics, Cell pyroptosis gene, Middle cerebral artery occlusion model, Pyroptosis genetic verification, Medicine, Biology (General), QH301-705.5 |
| الوصف: | Objective Cerebral infarction is the main cause of death in patients with cerebrovascular diseases. Our research aimed to screen and validate pyroptosis-related genes in cerebral infarction for the targeted therapy of cerebral infarction. Methods and results A total of 1,517 differentially expressed genes (DEGs) were obtained by DESeq2 software analysis. Gene set enrichment analysis results indicated that genes of middle cerebral artery occlusion (MCAO) mice aged 3 months and 18 months were enriched in pyroptosis, respectively. Differentially expressed pyroptosis-related genes (including Aim2, Casp8, Gsdmd, Naip2, Naip5, Naip6 and Trem2) were obtained through intersection of DEGs and genes from pyroptosis Gene Ontology Term (GO:0070269), and they were up-regulated in the brain tissues of MCAO mice in GSE137482. In addition, Casp8, Gsdmd, and Trem2 were verified to be significantly up-regulated in MCAO mice in GSE93376. The evaluation of neurologic function and triphenyltetrazolium chloride staining showed that the MCAO mouse models were successfully constructed. Meanwhile, the expressions of TNF-α, pyroptosis-related proteins, Casp8, Gsdmd and Trem2 in MCAO mice were significantly up-regulated. We selected Trem2 for subsequent functional analysis. OGD treatment of BV2 cell in vitro significantly upregulated the expressions of Trem2. Subsequent downregulation of Trem2 expression in OGD-BV2 cells further increased the level of pyroptosis. Therefore, Trem2 is a protective factor regulating pyroptosis, thus influencing the progression of cerebral infarction. Conclusions Casp8, Gsdmd and Trem2 can regulate pyroptosis, thus affecting cerebral infarction. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2167-8359 |
| العلاقة: | https://peerj.com/articles/16818.pdfTest; https://peerj.com/articles/16818Test/; https://doaj.org/toc/2167-8359Test |
| DOI: | 10.7717/peerj.16818 |
| الوصول الحر: | https://doaj.org/article/561083605b1c4efe9336a7744d025bcfTest |
| رقم الانضمام: | edsdoj.561083605b1c4efe9336a7744d025bcf |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21678359 |
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| DOI: | 10.7717/peerj.16818 |