Type 2-high asthma is associated with a specific indoor mycobiome and microbiome
| العنوان: | Type 2-high asthma is associated with a specific indoor mycobiome and microbiome |
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| المؤلفون: | Raphaël Enaud, Patrick Berger, Laurence Delhaes, Louise-Eva Vandenborght, Pierre-Olivier Girodet, Charlotte Urien, Stéphanie Ferreira, Noémie Coron |
| المصدر: | Europe PubMed Central The Journal of Allergy and Clinical Immunology |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Endotype, Exacerbation, V3-V4, Variable 3-variable 4 of the 16S rRNA encoding gene, microbiome, Severity of Illness Index, 0302 clinical medicine, Immunology and Allergy, Type 2 asthma, IL, Interleukin, OTU, Operational taxonomic unit, DNA, Fungal, medicine.diagnostic_test, ICS, Inhaled corticosteroid, T2, Type 2, Microbiota, Indoor environment, Dust, Middle Aged, ERS, European Respiratory Society, LABA, Long acting beta2 agonist, STB, Stable state, Air Pollution, Indoor, Female, medicine.symptom, EDC, Electrostatic Dust Collector, Environmental Monitoring, Spirometry, severe asthma, VRC, Voriconazole, Adult, DNA, Bacterial, BAL, Bronchoalveolar lavage, SA, Severe asthma, COBRA, Cohort of Bronchial obstruction and Asthma, Immunology, BMI, Body mass index, qPCR, Quantitative polymerase chain reaction, Article, EXA, Exacerbation of asthma, 03 medical and health sciences, mycobiome, medicine, ITS2, Internal transcribed spacer 2, Humans, Microbiome, FeNO, Asthma, Aged, business.industry, Sputum, medicine.disease, respiratory tract diseases, PERMANOVA, Permutational multivariate analysis of variance, 030104 developmental biology, 030228 respiratory system, NGS, Next generation sequencing, Metagenomics, PCoA, principal coordinates analysis, Exhaled nitric oxide, LAMA, Long acting muscarinic antagonist, OCS, Oral corticosteroid, business, FeNO, Fraction of exhaled nitric oxide, LDA, Linear discriminant analysis |
| الوصف: | Background The links between microbial environmental exposures and asthma are well documented, but no study has combined deep-sequencing results from pulmonary and indoor microbiomes of asthmatic patients with spirometry, clinical and endotype parameters. Objective The goal of this study was to investigate the links between indoor microbial exposures and pulmonary microbial communities and to document the role of microbial exposures on inflammatory and clinical outcomes of patients with severe asthma (SA). Methods Fifty-five SA patients from the national COBRA cohort were enrolled for analyzing their indoor microbial flora through the use of electrostatic dust collectors (EDCs). Among these patients, 22 were able to produce sputa during stable or pulmonary exacerbation periods and had complete pairs of EDC and sputum samples, both collected and analysed. We used amplicon targeted metagenomics to compare microbial communities from EDC and sputum samples of patients according to type 2 (T2)-asthma endotypes. Results Compared to patients with T2-low SA, patients with T2-high SA exhibited an increase in bacterial alpha-diversity and a decrease in fungal alpha-diversity of their indoor microbial floras, the latter being significantly correlated with FeNO levels. The beta-diversity of the EDC mycobiome significantly clustered according to T2 endotypes. Moreover, the proportion of fungal taxa in common between sputum and EDC samples was significantly higher when patients exhibited acute exacerbation. Conclusion These results illustrated, for the first time, a potential association between the indoor mycobiome and clinical features of SA patients, which should renew interest in deciphering the interactions between indoor environment, fungi, and host in asthma. Graphical abstract Capsule summary. This study is the first to date that investigates both the endogenous and the exogenous mycobiomes and microbiomes of asthmatic patients and reveals associations between indoor microbial communities, exacerbation, and T2 of severe asthma. |
| تدمد: | 1097-6825 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d25e9fb952c8b17fe7fdbe5e775803bTest https://pubmed.ncbi.nlm.nih.gov/33276993Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....4d25e9fb952c8b17fe7fdbe5e775803b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10976825 |
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