التفاصيل البيبلوغرافية
العنوان: |
Endothelial-derived extracellular vesicles induce cerebrovascular dysfunction in inflammation |
المؤلفون: |
D Roig-Carles (11574025), Eduard Willms (9491645), RD Fontijn (11574028), S Martinez-Pacheco (11574031), I Mäger (11574034), HE de Vries (11574037), M Hirst (11574040), B Sharrack (11574043), DK Male (11574046), CA Hawkes (11574049), IA Romero (11574052) |
سنة النشر: |
2021 |
المجموعة: |
Smithsonian Institution: Digital Repository |
مصطلحات موضوعية: |
Uncategorized, blood-brain barrier, cell-to-cell communication, exosomes, extracellular vesicles, neuroinflammation |
الوصف: |
Blood–brain barrier (BBB) dysfunction is a key hallmark in the pathology of many neu-roinflammatory disorders. Extracellular vesicles (EVs) are lipid membrane-enclosed carriers of molecular cargo that are involved in cell-to-cell communication. Circulating endothelial EVs are increased in the plasma of patients with neurological disorders, and immune cell-derived EVs are known to modulate cerebrovascular functions. However, little is known about whether brain endothelial cell (BEC)-derived EVs themselves contribute to BBB dysfunction. Human cerebral micro-vascular cells (hCMEC/D3) were treated with TNFα and IFNy, and the EVs were isolated and char-acterised. The effect of EVs on BBB transendothelial resistance (TEER) and leukocyte adhesion in hCMEC/D3 cells was measured by electric substrate cell-substrate impedance sensing and the flow-based T-cell adhesion assay. EV-induced molecular changes in recipient hCMEC/D3 cells were an-alysed by RT-qPCR and Western blotting. A stimulation of naïve hCMEC/D3 cells with small EVs (sEVs) reduced the TEER and increased the shear-resistant T-cell adhesion. The levels of microRNA-155, VCAM1 and ICAM1 were increased in sEV-treated hCMEC/D3 cells. Blocking the expression of VCAM1, but not of ICAM1, prevented sEV-mediated T-cell adhesion to brain endothelia. These results suggest that sEVs derived from inflamed BECs promote cerebrovascular dysfunction. These findings may provide new insights into the mechanisms involving neuroinflammatory disorders. |
نوع الوثيقة: |
article in journal/newspaper |
اللغة: |
unknown |
العلاقة: |
https://figshare.com/articles/journal_contribution/Endothelial-derived_extracellular_vesicles_induce_cerebrovascular_dysfunction_in_inflammation/16823800Test |
DOI: |
10.26181/16823800.v1 |
الإتاحة: |
https://doi.org/10.26181/16823800.v1Test |
حقوق: |
CC BY 4.0 |
رقم الانضمام: |
edsbas.45F59827 |
قاعدة البيانات: |
BASE |