Stigmasterols, ceramides, including ceramide-1-phosphates and pyrimidines were identified in an ethanol extract of the marine sponge Myxilla sp. The compounds were identified by MS and 1D- and 2D-NMR techniques. A simple method based on an ultra-high-performance liquid chromatography coupled with tandem mass spectrometry was developed to investigate the production of the aforementioned ceramides. This is the first report on ceramide-1-phosphates from a marine sponge.
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