دورية أكاديمية
Regulatory Effect of Cannabidiol (CBD) on Decreased β-Catenin Expression in Alopecia Models by Testosterone and PMA Treatment in Dermal Papilla Cells
| العنوان: | Regulatory Effect of Cannabidiol (CBD) on Decreased β-Catenin Expression in Alopecia Models by Testosterone and PMA Treatment in Dermal Papilla Cells |
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| المؤلفون: | Yoon-Jong Park, Jae-Min Ryu, Han-Heom Na, Hyun-Suk Jung, Bokhye Kim, Jin-Sung Park, Byung-Soo Ahn, Keun-Cheol Kim |
| المصدر: | Journal of Pharmacopuncture, Vol 24, Iss 2, Pp 68-75 (2021) |
| بيانات النشر: | Korean Pharmacopuncture Institute, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Medicine LCC:Miscellaneous systems and treatments LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | alopecia, dermal papilla, wnt/β-catenin, testosterone, phorbol 12-myristate 13-acetate (pma), cannabidiol (cbd), Medicine, Miscellaneous systems and treatments, RZ409.7-999, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Objectives: The hair follicle is composed of more than 20 kinds of cells, and mesoderm derived dermal papilla cells and keratinocytes cooperatively contribute hair growth via Wnt/β-catenin signaling pathway. We are to investigate β-catenin expression and regulatory mechanism by CBD in alopecia hair tissues and dermal papilla cells. Methods : : We performed structural and anatomical analyses on alopecia patients derived hair tissues using microscopes. Pharmacological effect of CBD was evaluated by β-catenin expression using RT-PCR and immunostaining experiment. Results : : Morphological deformation and loss of cell numbers in hair shaft were observed in alopecia hair tissues. IHC experiment showed that loss of β-catenin expression was shown in inner shaft of the alopecia hair tissues, indicating that β-catenin expression is a key regulatory function during alopecia progression. Consistently, β-catenin expression was decreased in testosterone or PMA treated dermal papilla cells, suggesting that those treatments are referred as a model on molecular mechanism of alopecia using dermal papilla cells. RT-PCR and immunostaining experiments showed that β-catenin expression was decreased in RNA level, as well as decreased β-catenin protein might be resulted from ubiquitination. However, CBD treatment has no changes in gene expression including β-catenin, but the decreased β-catenin expression by testosterone or PMA was restored by CBD pretreatment, suggesting that potential regulatory effect on alopecia induction of testosterone and PMA. Conclusion : : CBD might have a modulating function on alopecia caused by hormonal or excess of signaling pathway, and be a promising application for on alopecia treatment. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Korean |
| تدمد: | 2093-6966 |
| العلاقة: | https://www.journal-jop.org/journal/view.html?doi=10.3831/KPI.2021.24.2.68Test; https://doaj.org/toc/2093-6966Test |
| DOI: | 10.3831/KPI.2021.24.2.68 |
| الوصول الحر: | https://doaj.org/article/9d7e431d091e4b19acff64248bf7142eTest |
| رقم الانضمام: | edsdoj.9d7e431d091e4b19acff64248bf7142e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20936966 |
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| DOI: | 10.3831/KPI.2021.24.2.68 |