دورية أكاديمية
Immune Profiling Panel: A Proof-of-Concept Study of a New Multiplex Molecular Tool to Assess the Immune Status of Critically Ill Patients
| العنوان: | Immune Profiling Panel: A Proof-of-Concept Study of a New Multiplex Molecular Tool to Assess the Immune Status of Critically Ill Patients |
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| المؤلفون: | Tawfik, Dina M, Vachot, Laurence, Bocquet, Adeline, Venet, Fabienne, Rimmelé, Thomas, Monneret, Guillaume, Blein, Sophie, Montgomery, Jesse L, Hemmert, Andrew C, Pachot, Alexandre, Moucadel, Virginie, Yugueros-Marcos, Javier, Brengel-Pesce, Karen, Mallet, François, Textoris, Julien |
| المساهمون: | bioMérieux, Hospices Civils de Lyon, European Union’s Horizon-2020, Marie Skłodowska-Curie Innovative Training Networks |
| المصدر: | The Journal of Infectious Diseases ; volume 222, issue Supplement_2, page S84-S95 ; ISSN 0022-1899 1537-6613 |
| بيانات النشر: | Oxford University Press (OUP) |
| سنة النشر: | 2020 |
| الوصف: | Background Critical illness such as sepsis is a life-threatening syndrome defined as a dysregulated host response to infection and is characterized by patients exhibiting impaired immune response. In the field of diagnosis, a gap still remains in identifying the immune profile of critically ill patients in the intensive care unit (ICU). Methods A new multiplex immune profiling panel (IPP) prototype was assessed for its ability to semiquantify messenger RNA immune-related markers directly from blood, using the FilmArray System, in less than an hour. Samples from 30 healthy volunteers were used for the technical assessment of the IPP tool. Then the tool was clinically assessed using samples from 10 healthy volunteers and 20 septic shock patients stratified using human leukocyte antigen–DR expression on monocytes (mHLA-DR). Results The IPP prototype consists of 16 biomarkers that target the immune response. The majority of the assays had a linear expression with different RNA inputs and a coefficient of determination (R2) > 0.8. Results from the IPP pouch were comparable to standard quantitative polymerase chain reaction and the assays were within the limits of agreement in Bland–Altman analysis. Quantification cycle values of the target genes were normalized against reference genes and confirmed to account for the different cell count and technical variability. The clinical assessment of the IPP markers demonstrated various gene modulations that could distinctly differentiate 3 profiles: healthy volunteers, intermediate mHLA-DR septic shock patients, and low mHLA-DR septic shock patients. Conclusions The use of IPP showed great potential for the development of a fully automated, rapid, and easy-to-use immune profiling tool. The IPP tool may be used in the future to stratify critically ill patients in the ICU according to their immune status. Such stratification will enable personalized management of patients and guide treatments to avoid secondary infections and lower mortality. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| DOI: | 10.1093/infdis/jiaa248 |
| الإتاحة: | https://doi.org/10.1093/infdis/jiaa248Test http://academic.oup.com/jid/article-pdf/222/Supplement_2/S84/33515954/jiaa248.pdfTest |
| حقوق: | http://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.E245AD80 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/infdis/jiaa248 |
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