التفاصيل البيبلوغرافية
| العنوان: |
A Portable Diagnostic Assay, Genetic Diversity, and Isolation of Seoul Virus from Rattus norvegicus Collected in Gangwon Province, Republic of Korea |
| المؤلفون: |
Kyungmin Park, Seung-Ho Lee, Jongwoo Kim, Jingyeong Lee, Geum-Young Lee, Seungchan Cho, Juyoung Noh, Jeewan Choi, Juwon Park, Dong-Hyun Song, Se Hun Gu, Hyeongseok Yun, Jung-Eun Kim, Daesang Lee, Il-Ung Hwang, Won-Keun Kim, Jin-Won Song |
| المصدر: |
Pathogens; Volume 11; Issue 9; Pages: 1047 |
| بيانات النشر: |
Multidisciplinary Digital Publishing Institute |
| سنة النشر: |
2022 |
| المجموعة: |
MDPI Open Access Publishing |
| مصطلحات موضوعية: |
Seoul virus, hemorrhagic fever with renal syndrome, Biomeme system, portable diagnostic assay, molecular diagnosis, next-generation sequencing, whole-genome sequencing, genetic diversity, hantavirus isolation |
| جغرافية الموضوع: |
agris |
| الوصف: |
Seoul virus (SEOV), an etiological agent for hemorrhagic fever with renal syndrome, poses a significant public health threat worldwide. This study evaluated the feasibility of a mobile Biomeme platform for facilitating rapid decision making of SEOV infection. A total of 27 Rattus norvegicus were collected from Seoul Metropolitan City and Gangwon Province in Republic of Korea (ROK), during 2016–2020. The serological and molecular prevalence of SEOV was 5/27 (18.5%) and 2/27 (7.4%), respectively. SEOV RNA was detected in multiple tissues of rodents using the Biomeme device, with differences in Ct values ranging from 0.6 to 2.1 cycles compared to a laboratory benchtop system. Using amplicon-based next-generation sequencing, whole-genome sequences of SEOV were acquired from lung tissues of Rn18-1 and Rn19-5 collected in Gangwon Province. Phylogenetic analysis showed a phylogeographical diversity of rat-borne orthohantavirus collected in Gangwon Province. We report a novel isolate of SEOV Rn19-5 from Gangwon Province. Our findings demonstrated that the Biomeme system can be applied for the molecular diagnosis of SEOV comparably to the laboratory-based platform. Whole-genome sequencing of SEOV revealed the phylogeographical diversity of orthohantavirus in the ROK. This study provides important insights into the field-deployable diagnostic assays and genetic diversity of orthohantaviruses for the rapid response to hantaviral outbreaks in the ROK. |
| نوع الوثيقة: |
text |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
Viral Pathogens; https://dx.doi.org/10.3390/pathogens11091047Test |
| DOI: |
10.3390/pathogens11091047 |
| الإتاحة: |
https://doi.org/10.3390/pathogens11091047Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.7743FC6C |
| قاعدة البيانات: |
BASE |
