| الوصف: |
The aim of this study was to verify the existence of possible cross-talk between biochemical contents of follicular fluid (FF) and systemic concentrations according to the follicular development of the metabolites: glucose (GLU), lactate (LACT), cholesterol (CHOL), triglycerides (TG), total bilirubin (T BIL), blood urea nitrogen (BUN), and creatinine (CREAT); enzymatic activities: gamma-glutamyl transferase (GGT), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and aspartate aminotransferase (AST); electrolytes: calcium (Ca), phosphorus (P), sodium (Na), chloride (Cl), potassium (K), magnesium (Mg), and iron (Fe); total proteins (TP) and their fractions: albumin (ALB), α1-, α2-, β-, and γ-globulins (GLOB) of FF and blood strain and their correlations with follicular size in cycling mares. Systemic concentrations of GLU, T BIL, BUN, Fe, TP, ALB, α-1, and α-2 and δ-GLOB and of ALP, GGT, and AST activities were higher than in the FF (P.05); LACT, CHOL, and TG were higher in FF than systemic ones (P.05). Glucose, CHOL, TG, LACT, and T BIL were higher in large follicles than in medium and small follicles (P.05); however, BUN, Fe, ALP, and AST were lower in large follicles than in medium or small follicles (P.05). Alkaline phosphatase, GGT, and AST activities decreased in medium and large follicles compared with small follicles (P.05). These results suggest that the metabolic, enzymatic, electrolytic, and protein composition of FF of growing follicles could occur according to the bloodstream changes; hence, it is possible to presume that the nutritional environment of oocyte and follicular cells could improve the clinical diagnoses of infertility in the mare. |
