دورية أكاديمية
Whole-slide image analysis identifies a high content of Hodgkin Reed-Sternberg cells and a low content of T lymphocytes in tumor microenvironment as predictors of adverse outcome in patients with classic Hodgkin lymphoma treated with ABVD
العنوان: | Whole-slide image analysis identifies a high content of Hodgkin Reed-Sternberg cells and a low content of T lymphocytes in tumor microenvironment as predictors of adverse outcome in patients with classic Hodgkin lymphoma treated with ABVD |
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المؤلفون: | Santiesteban Espejo, Antonio, Bernal-Florido, Irene, Pérez-Requena, José, Atienza Cuevas, Lidia, Maira González, Nieves, García-Rojo, Marcial |
المساهمون: | Anatomía Patológica, Biología Celular, Histología, Historia de la Ciencia, Medicina Legal y Forense y Toxicología |
المصدر: | Frontiers in Oncology, Vol. 12, 2022 |
بيانات النشر: | FRONTIERS MEDIA SA |
سنة النشر: | 2023 |
المجموعة: | RODIN - Repositorio de Objetos de Docencia e Investigación de la Universidad de Cádiz |
مصطلحات موضوعية: | whole-slide imaging, classic Hodgkin lymphoma, digital image analysis, tumor microenvironment, Hodgkin Reed-Sternberg |
الوصف: | Classic Hodgkin lymphoma (cHL) constitutes the most frequent lymphoma in young adults. Its histopathology is unique as a scattered tumor population, termed Hodgkin Reed-Sternberg (HRS) cells is diluted in a prominent tumor microenvironment (TME) composed of T lymphocytes, B lymphocytes, macrophages, neutrophils, eosinophils and histiocytes. Traditionally, the identification of prognostic biomarkers in the cHL TME has required visual inspection and manual counting by pathologists. The advent of whole-slide imaging (WSI) and digital image analysis methods could significantly contribute to improve this essential objective in cHL research, as a 10-20% of patients are still refractory or relapsed after conventional chemotherapy. In this work, we have digitized a total of 255 diagnostic cHL slides and quantified the proportion of HRS cells (CD30), B cells (CD20) and T cells (CD3) by digital image analysis. Data obtained where then correlated with the overall survival (OS) and progression free survival (PFS) of cHL patients. Quantification of HRS cells, B cells and T cells reflects the biological heterogeneity of the different cHL histological subtypes analyzed. A percentage of 2.00% of HRS cells statistically significantly discriminated between patients achieving a complete metabolic response (CMR) and refractory or relapsed (R/R) patients both for the OS (P=0.001) and PFS (P=0.005). Furthermore, patients with a percentage of T cells below the 26.70% in the TME showed a statistically significantly shorter OS (P=0.019) and PFS (P=0.041) in comparison with patients above this threshold. A subgroup of patients with a low content of T cells and high content of HRS cells exhibited a special aggressive clinical course. Currently, there is the need to implement quantitative and easy scalable methods to enhance clinical translation, as the cHL TME plays a central role in the clinical course of the disease. The results of this study could contribute to the identification of prognostic biomarkers specifically looking at the cHL ... |
نوع الوثيقة: | article in journal/newspaper |
وصف الملف: | application/pdf |
اللغة: | English |
تدمد: | 2234-943X |
العلاقة: | info:eu-repo/grantAgreement/Junta de Andalucía//PI0032-2017; http://hdl.handle.net/10498/28799Test |
DOI: | 10.3389/fonc.2022.1000762 |
الإتاحة: | https://doi.org/10.3389/fonc.2022.1000762Test http://hdl.handle.net/10498/28799Test |
حقوق: | Atribución 4.0 Internacional ; http://creativecommons.org/licenses/by/4.0Test/ ; open access |
رقم الانضمام: | edsbas.E7997439 |
قاعدة البيانات: | BASE |
تدمد: | 2234943X |
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DOI: | 10.3389/fonc.2022.1000762 |