التفاصيل البيبلوغرافية
| العنوان: |
Establishment of a mouse allergy model for Culicoides (Diptera: Ceratopogonidae) |
| المؤلفون: |
Bao, Shuncai, Lu, Xue, Xiang, Shuai, Hou, Xiaohui |
| المساهمون: |
National Natural Science Foundation of China |
| المصدر: |
Veterinary Medicine and Science ; volume 10, issue 3 ; ISSN 2053-1095 2053-1095 |
| بيانات النشر: |
Wiley |
| سنة النشر: |
2024 |
| المجموعة: |
Wiley Online Library (Open Access Articles via Crossref) |
| الوصف: |
Background Culicoides is a genus of ubiquitous biting midges (Ceratopogonidae). Female midges have blood‐sucking habit. They not only bite and harass humans and animals but also may be an important vector of disease transmission. Therefore, building an animal allergy model caused by Culicoides biting is very beneficial for studying its pathogenesis and exploring the therapeutic methods. Material and method Kunming mice were used in this study to build the model and sensitised by two‐step injection of midge extracts. Scratching behaviour and histological examination were used to check the immediate and delayed responses. Immunoglobulin E (IgE) and Immunoglobulin G (IgG) were detected using indirect enzyme‐linked immunosorbent assay (ELISA) assay. Splenic cell proliferation and cytokine production were determined using 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyl tetrazolium bromide (MTT) and ELISA assays. The response of cytokine gene expression to midge stimulation was analysed through quantitative real‐time polymerase chain reaction (qPCR). Results Behavioural results revealed a significant increase in scratching frequency among the midge‐sensitised animals ( p < 0.05). Histological examination showed more inflammatory cytokine infiltration at the injection site of midge‐sensitised mice comparing to the ones in the control group. The serum levels of IgE and IgG1 antibodies in the midge‐sensitised group were significantly elevated ( p < 0.05). After splenocytes were stimulated in vitro with midge extracts, the midge‐sensitised group's splenocyte count significantly increased in comparison to the control group. The midge‐sensitised group's qPCR data revealed a down‐regulation of tumor necrosis factor alpha (TNF‐α) expression and an increase in the expression of interleukin (IL)‐4, IL‐5, IL‐10 and IL‐13 but not in the control group ( p < 0.05). Conclusions In this study, an animal model of Culicoides –mouse sensitisation was successfully constructed using a two‐step method. The mode of ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| DOI: |
10.1002/vms3.1462 |
| الإتاحة: |
https://doi.org/10.1002/vms3.1462Test |
| حقوق: |
http://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
| رقم الانضمام: |
edsbas.A0B19E1D |
| قاعدة البيانات: |
BASE |
