دورية أكاديمية
Shining a spotlight on methyl groups: photochemically induced dynamic nuclear polarization spectroscopy of 5-Deazariboflavin and its nor analogs
| العنوان: | Shining a spotlight on methyl groups: photochemically induced dynamic nuclear polarization spectroscopy of 5-Deazariboflavin and its nor analogs |
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| المؤلفون: | Panter, Sabrina, Ayekoi, Audrey, Tesche, Jannis, Chen, Jing, Illarionov, Boris, Bacher, Adelbert, Fischer, Markus, Weber, Stefan |
| المصدر: | International journal of molecular sciences. - 25, 2 (2024) , 848, ISSN: 1422-0067 |
| سنة النشر: | 2024 |
| المجموعة: | University of Freiburg: FreiDok |
| الوصف: | 5-Deazaflavins are analogs of naturally occurring flavin cofactors. They serve as substitutes for natural flavin cofactors to investigate and modify the reaction pathways of flavoproteins. Demethylated 5-deazaflavins are potential candidates for artificial cofactors, allowing us to fine-tune the reaction kinetics and absorption characteristics of flavoproteins. In this contribution, demethylated 5-deazariboflavin radicals are investigated (1) to assess the influence of the methyl groups on the electronic structure of the 5-deazaflavin radical and (2) to explore their photophysical properties with regard to their potential as artificial cofactors. We determined the proton hyperfine structure of demethylated 5-deazariboflavins using photochemically induced dynamic nuclear polarization (photo-CIDNP) spectroscopy, as well as density functional theory (DFT). To provide context, we compare our findings to a study of flavin mononucleotide (FMN) derivatives. We found a significant influence of the methylation pattern on the absorption properties, as well as on the proton hyperfine coupling ratios of the xylene moiety, which appears to be solvent-dependent. This effect is enhanced by the replacement of N5 by C5-H in 5-deazaflavin derivatives compared to their respective flavin counterparts. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | |
| اللغة: | English |
| العلاقة: | https://freidok.uni-freiburg.de/data/243281Test |
| DOI: | 10.3390/ijms25020848 |
| الإتاحة: | https://doi.org/10.3390/ijms25020848Test https://freidok.uni-freiburg.de/data/243281Test https://nbn-resolving.org/urn:nbn:de:bsz:25-freidok-2432812Test https://freidok.uni-freiburg.de/dnb/download/243281Test |
| حقوق: | free |
| رقم الانضمام: | edsbas.B4B03139 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/ijms25020848 |
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