MMP-1 activation contributes to airway smooth muscle growth and asthma severity
| العنوان: | MMP-1 activation contributes to airway smooth muscle growth and asthma severity |
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| المؤلفون: | Naveed, SU, Clements, D, Jackson, DJ, Philp, C, Billington, CK, Soomro, I, Reynolds, C, Harrison, TW, Johnston, SL, Shaw, DE, Johnson, SR |
| المساهمون: | Commission of the European Communities, Asthma UK, Medical Research Council (MRC), National Institute for Health Research |
| بيانات النشر: | American Thoracic Society, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Adult, Male, extracellular matrix, Respiratory System, Myocytes, Smooth Muscle, Cell Culture Techniques, mast cells, Bronchi, Severity of Illness Index, Bronchial Provocation Tests, airway remodeling, Critical Care Medicine, INFLAMMATION, General & Internal Medicine, EXTRACELLULAR-MATRIX, Humans, Asthma, Extracellular matrix, Airway remodeling, Airway smooth muscle, Mast cells, Science & Technology, BRONCHOCONSTRICTION, PROLIFERATION, CHILDHOOD ASTHMA, Muscle, Smooth, IN-VITRO, 11 Medical And Health Sciences, asthma, respiratory system, airway smooth muscle, respiratory tract diseases, Spirometry, SERINE PROTEASES, MAST-CELLS, Female, OBSTRUCTION, Bronchial Hyperreactivity, Matrix Metalloproteinase 1, COLLAGENASE, Life Sciences & Biomedicine |
| الوصف: | INTRODUCTION: Matrix metalloproteinase-1 and mast cells are present in the airways of people with asthma. We hypothesised that matrix metalloproteinase-1 could be activated by mast cells and increase asthma severity. METHODS: Patients with stable asthma and healthy controls underwent spirometry, methacholine challenge, bronchoscopy and their airway smooth muscle cells were grown in culture. A second asthma group and controls had symptom scores, spirometry and bronchoalveolar lavage before and after rhinovirus-induced asthma exacerbations. Extra-cellular matrix was prepared from decellularised airway smooth muscle cultures. Matrix metalloproteinase-1 protein and activity were assessed. RESULTS: Airway smooth muscle cells generated pro-matrix metalloproteinase-1 which was proteolytically activated by mast cell tryptase. Airway smooth muscle treated with activated mast cell supernatants produced extra-cellular matrix which enhanced subsequent airway smooth muscle growth by 1.5 fold (p |
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| اللغة: | English |
| تدمد: | 1073-449X 1535-4970 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=dedup_wf_001::bb8d6e755f2bb33a5d278093a6e84518Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.dedup.wf.001..bb8d6e755f2bb33a5d278093a6e84518 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 1073449X 15354970 |
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