التفاصيل البيبلوغرافية
| العنوان: |
Comprehensive analysis of brain injury parameters in a preclinical porcine model of acute liver failure |
| المؤلفون: |
Felgendreff, Philipp, Hosseiniasl, Seyed M., Felgendreff, Lisa, Amiot, Bruce P., Minshew, Anna, Ahmadzada, Boyukkhanim, Qu, Zhi, Wilken, Silvana, Arribas Gomez, Ines, Nyberg, Scott L., Cook, Casey N. |
| المصدر: |
Frontiers in Medicine ; volume 11 ; ISSN 2296-858X |
| بيانات النشر: |
Frontiers Media SA |
| سنة النشر: |
2024 |
| المجموعة: |
Frontiers (Publisher - via CrossRef) |
| مصطلحات موضوعية: |
General Medicine |
| الوصف: |
Introduction Acute liver failure (ALF) is defined as acute loss of liver function leading to hepatic encephalopathy associated with a high risk of patient death. Brain injury markers in serum and tissue can help detect and monitor ALF-associated brain injury. This study compares different brain injury parameters in plasma and tissue along with the progression of ALF. Method ALF was induced by performing an 85% liver resection. Following the resection, animals were recovered and monitored for up to 48 h or until reaching the predefined endpoint of receiving standard medical therapy (SMT). Blood and serum samples were taken at T baseline , T 24 , and upon reaching the endpoint (T end ). Control animals were euthanized by exsanguination following plasma sampling. Postmortem brain tissue samples were collected from the frontal cortex (FCTx) and cerebellum (Cb) of all animals. Glial fibrillary acidic protein (GFAP) and tau protein and mRNA levels were quantified using ELISA and qRT-PCR in all plasma and brain samples. Plasma neurofilament light (NFL) was also measured using ELISA. Results All ALF animals ( n = 4) were euthanized upon showing signs of brain herniation. Evaluation of brain injury biomarkers revealed that GFAP was elevated in ALF animals at T 24h and T end , while Tau and NFL concentrations were unchanged. Moreover, plasma glial fibrillary acidic protein (GFAP) levels were negatively correlated with total protein and positively correlated with both aspartate transaminase (AST) and alkaline phosphatase (AP). Additionally, lower GFAP and tau RNA expressions were observed in the FCTx of the ALF group but not in the CB tissue. Conclusion The current large animal study has identified a strong correlation between GFAP concentration in the blood and markers of ALF. Additionally, the protein and gene expression analyses in the FCTx revealed that this area appears to be susceptible, while the CB is protected from the detrimental impacts of ALF-associated brain swelling. These results warrant further studies to ... |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| DOI: |
10.3389/fmed.2024.1363979 |
| DOI: |
10.3389/fmed.2024.1363979/full |
| الإتاحة: |
https://doi.org/10.3389/fmed.2024.1363979Test |
| حقوق: |
https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: |
edsbas.71DB7096 |
| قاعدة البيانات: |
BASE |
