دورية أكاديمية
Angiogenic activity of human chorionic gonadotropin through LH receptor activation on endothelial and epithelial cells of the endometrium
| العنوان: | Angiogenic activity of human chorionic gonadotropin through LH receptor activation on endothelial and epithelial cells of the endometrium |
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| المؤلفون: | Berndt, Sarah, PERRIER d'HAUTERIVE, Sophie, Blacher, Silvia, Pequeux, Christel, Lorquet, Sophie, Munaut, Carine, Applanat, Martinne, Herve, M. A., Lamande, N., Corvol, P., van den Brule, Frederic, Frankenne, Francis, Poutanen, M., Huhtaniemi, I., Geenen, Vincent, Noël, Agnès, Foidart, Jean-Michel |
| المصدر: | FASEB Journal, 20 (14), 2630-2632 (2006-12) |
| بيانات النشر: | Federation of American Society for Experimental Biology |
| سنة النشر: | 2006 |
| المجموعة: | University of Liège: ORBi (Open Repository and Bibliography) |
| مصطلحات موضوعية: | angiogenesis, hCG, Life sciences, Biochemistry, biophysics & molecular biology, Sciences du vivant, Biochimie, biophysique & biologie moléculaire |
| الوصف: | peer reviewed ; Successful embryo development requires an extensive endometrial angiogenesis in proximity of implantation site. The glycoprotein hCG is produced even before implantation by trophoblast in normal pregnancy. In this manuscript, we demonstrate an angiogenic effect of hCG in several in vivo (chick chorioallantoic membrane, matrigel plug assay, aortic ring assay) and in vitro experimental models. In contrast, human placental lactogen (hPL) did not display angiogenic properties. LH/hCG receptor was detected in endothelial cells by reverse-transcriptase polymerase chain reaction (RT-PCR) and by Western blotting. In mice aortic ring assay, angiostimulation by hCG was abrogated by deletion of LH/hCG receptor (LuRKO mice). Use of recombinant hCG and anti-hCG antibody (Ab) further confirmed the specificity of this angiogenic activity. By using dibutyryl cAMP, adenylate cyclase, or protein kinase A inhibitors, we demonstrate that hCG-mediated angiogenesis involves adenylyl-cyclase-protein kinase A activation. Addition of hCG to endometrial epithelial epithelial cells, but not to cultured endothelial cells, stimulated vascular endothelial growth factor (VEGF). VEGF and hCG also displayed additive activities. Altogether, these data demonstrate that peritrophoblastic angiostimulation may result from a paracrine dialogue between trophoblast, epithelial, and endothelial cells through hCG and VEGF. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0892-6638 1530-6860 |
| العلاقة: | urn:issn:0892-6638; urn:issn:1530-6860; https://orbi.uliege.be/handle/2268/9262Test; info:hdl:2268/9262; scopus-id:2-s2.0-33845662625; info:pmid:17065221 |
| DOI: | 10.1096/fj.06-5885fje |
| الإتاحة: | https://doi.org/10.1096/fj.06-5885fjeTest https://orbi.uliege.be/handle/2268/9262Test |
| حقوق: | restricted access ; http://purl.org/coar/access_right/c_16ecTest ; info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.9F673D80 |
| قاعدة البيانات: | BASE |
| تدمد: | 08926638 15306860 |
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| DOI: | 10.1096/fj.06-5885fje |