دورية أكاديمية
Development of a Probability-Based In Vitro Eye Irritation Screening Platform
| العنوان: | Development of a Probability-Based In Vitro Eye Irritation Screening Platform |
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| المؤلفون: | Seep Arora, Anna Goralczyk, Sujana Andra, Soon Yew John Lim, Yi-Chin Toh |
| المصدر: | Bioengineering, Vol 11, Iss 4, p 315 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Technology LCC:Biology (General) |
| مصطلحات موضوعية: | eye irritation test, cell micropatterning, TRPV1 receptor, nociception, 3Rs tool, Technology, Biology (General), QH301-705.5 |
| الوصف: | Traditional eye irritation assessments, which rely on animal models or ex vivo tissues, face limitations due to ethical concerns, costs, and low throughput. Although numerous in vitro tests have been developed, none have successfully reconciled the need for high experimental throughput with the accurate prediction of irritation potential, attributable to the complexity of irritation mechanisms. Simple cell models, while suitable for high-throughput screening, offer limited mechanistic insights, contrasting with more physiologically relevant but less scalable complex organotypic corneal tissue constructs. This study presents a novel strategy to enhance the predictive accuracy of screening-compatible simple cell models in eye irritation testing. Our method combines the results of two in vitro assays—cell apoptosis and nociceptor (TRPV1) activation—using micropatterned chips to partition human corneal epithelial cells into numerous discrete small populations. Following exposure to test compounds, we measure apoptosis and nociceptor activation responses. The large datasets collected from the cell micropatterns facilitate binarization and statistical fitting to calculate a mathematical probability, which assesses the compound’s potential to cause eye irritation. This method potentially enables the amalgamation of multiple mechanistic readouts into a singular index, providing a more accurate and reliable prediction of eye irritation potential in a format amenable to high-throughput screening. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2306-5354 |
| العلاقة: | https://www.mdpi.com/2306-5354/11/4/315Test; https://doaj.org/toc/2306-5354Test |
| DOI: | 10.3390/bioengineering11040315 |
| الوصول الحر: | https://doaj.org/article/177f71d225ee42e2ac8b8b9f55bfaf0dTest |
| رقم الانضمام: | edsdoj.177f71d225ee42e2ac8b8b9f55bfaf0d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23065354 |
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| DOI: | 10.3390/bioengineering11040315 |