التفاصيل البيبلوغرافية
| العنوان: |
Anti-P 200 pemphigoid - The most common floor binding subepidermal autoimmune bullous disease in a tertiary care center in south India. |
| المؤلفون: |
Rai, Reena1, Anand, J. Bede1 Yboy.bede@gmail.com, Shanmugasekar, C.1, Arunprasath, P.1, Chaitra, V.2, Zillikens, Detlef3, Schimdt, Enno3 |
| المصدر: |
Indian Journal of Dermatology, Venereology & Leprology. Nov/Dec2021, Vol. 87 Issue 6, p1-5. 5p. |
| مصطلحات موضوعية: |
*BULLOUS pemphigoid, *AUTOIMMUNE diseases, *TERTIARY care, *EPIDERMOLYSIS bullosa, *IMMUNOFLUORESCENCE, *HOSPITAL patients, *AUTOANTIBODIES, *SPECIALTY hospitals, *CROSS-sectional method, *MICROSCOPY, *RETROSPECTIVE studies, *FLUORESCENT antibody technique |
| مصطلحات جغرافية: |
INDIA |
| مستخلص: |
Background: The pemphigoid group of diseases may present clinically and immunologically in a very similar fashion. Indirect immunofluorescence microscopy with readily available salt-split human skin in a BIOCHIP™ helps to classify these conditions as those with either with roof binding or floor binding of immunoreactants. Epidermolysis bullosa acquisita, anti-laminin 332 pemphigoid and anti-p200 pemphigoid show floor binding, while in the most frequent type of pemphigoid disease, bullous pemphigoid, epidermal side staining pattern is seen on salt-split skin Aims: The aim of the study was to detect the target antigens in sub-epidermal bullous diseases.Methods: Forty patients with bullous pemphigoid diagnosed by lesional histopathology and direct immunofluorescence microscopy were re-evaluated by a BIOCHIP™ mosaic containing both tissue substrates and recombinant target antigens. Sera with floor pattern staining on salt-split skin were further evaluated by immunoblotting with dermal extract.Results: Five patients with floor staining had anti-p200 pemphigoid.Limitations: We could not perform serration pattern analysis of direct immunofluorescence in our patients.Conclusion: Histopathology and direct immunofluorescence microscopy cannot differentiate between various entities of pemphigoid diseases. A multivariant approach using a BIOCHIP™ mosaic including salt-split skin followed by immunoblotting with dermal extract helps to identify the target antigen. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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