التفاصيل البيبلوغرافية
| العنوان: |
A Semi-Quantitative Isothermal Diagnostic Assay Utilizing Competitive Amplification |
| المؤلفون: |
Christopher P. Mancuso (11036938), Zhi-Xiang Lu (7289087), Jason Qian (11036941), Sarah A. Boswell (11036944), Michael Springer (37212) |
| سنة النشر: |
2021 |
| المجموعة: |
Smithsonian Institution: Digital Repository |
| مصطلحات موضوعية: |
Biophysics, Biochemistry, Genetics, Molecular Biology, Pharmacology, Biotechnology, Immunology, Infectious Diseases, Mathematical Sciences not elsewhere classified, Chemical Sciences not elsewhere classified, DNA, HIV, gold-standard techniques, treatment strategies, RPA, Amplification Quantitative diagnostics, Semi-Quantitative Isothermal Diagno., field-deployable assays, target analyte, RNA, COVID -19 patient samples, semi-quantitative information, recombinase polymerase amplification, qRPA |
| الوصف: |
Quantitative diagnostics that are rapid, inexpensive, sensitive, robust, and field-deployable are needed to contain the spread of infectious diseases and inform treatment strategies. While current gold-standard techniques are highly sensitive and quantitative, they are slow and require expensive equipment. Conversely, current rapid field-deployable assays available provide essentially binary information about the presence of the target analyte, not a quantitative measure of concentration. Here, we report the development of a molecular diagnostic test [quantitative recombinase polymerase amplification (qRPA)] that utilizes competitive amplification during a recombinase polymerase amplification (RPA) assay to provide semi-quantitative information on a target nucleic acid. We demonstrate that qRPA can quantify DNA, RNA, and viral titers in HIV and COVID-19 patient samples and that it is more robust to environmental perturbations than traditional RPA. These features make qRPA potentially useful for at-home testing to monitor the progress of viral infections or other diseases. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
unknown |
| العلاقة: |
https://figshare.com/articles/journal_contribution/A_Semi-Quantitative_Isothermal_Diagnostic_Assay_Utilizing_Competitive_Amplification/14866873Test |
| DOI: |
10.1021/acs.analchem.1c01576.s001 |
| الإتاحة: |
https://doi.org/10.1021/acs.analchem.1c01576.s001Test |
| حقوق: |
CC BY-NC 4.0 |
| رقم الانضمام: |
edsbas.C67C1881 |
| قاعدة البيانات: |
BASE |
