دورية أكاديمية
Antibacterial and Antihemolytic Activity of New Biomaterial Based on Glycyrrhizic Acid and Quercetin (GAQ) against Staphylococcus aureus
العنوان: | Antibacterial and Antihemolytic Activity of New Biomaterial Based on Glycyrrhizic Acid and Quercetin (GAQ) against Staphylococcus aureus |
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المؤلفون: | Ewa Olchowik-Grabarek, Krzysztof Czerkas, Alimjon Davletboevich Matchanov, Rahmat Sulton Esanov, Umarbek Davlatboevich Matchanov, Maria Zamaraeva, Szymon Sekowski |
المصدر: | Journal of Functional Biomaterials, Vol 14, Iss 7, p 368 (2023) |
بيانات النشر: | MDPI AG, 2023. |
سنة النشر: | 2023 |
المجموعة: | LCC:Biotechnology LCC:Medicine (General) |
مصطلحات موضوعية: | quercetin, glycyrrhizic acid, α-hemolysin, Staphylococcus aureus, erythrocytes, membrane fluidity, Biotechnology, TP248.13-248.65, Medicine (General), R5-920 |
الوصف: | The goal of this study is to obtain and characterize the complex of quercetin with glycyrrhizic acid, which is known to serve as a drug delivery system. Quercetin is a flavonoid with a wide range of biological activities, including an antimicrobial effect. However, quercetin instability and low bioavailability that limits its use in medical practice makes it necessary to look for new nanoformulations of it. The formation of the GAQ complex (2:1) was confirmed by using UV and FT-IR spectroscopies. It was found that the GAQ exhibited antimicrobial and antihemolytical activities against S. aureus bacteria and its main virulent factor—α-hemolysin. The IC50 value for the antihemolytical effect of GAQ was 1.923 ± 0.255 µg/mL. Using a fluorescence method, we also showed that the GAQ bound tightly to the toxin that appears to underlie its antihemolytic activity. In addition, another mechanism of the antihemolytic activity of the GAQ against α-hemolysin was shown, namely, its ability to increase the rigidity of the outer layer of the erythrocyte membrane and thus inhibit the incorporation of α-hemolysin into the target cells, increasing their resistance to the toxin. Both of these effects of GAQ were observed at concentrations below the MIC value for S. aureus growth, indicating the potential of the complex as an antivirulence agent. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2079-4983 |
العلاقة: | https://www.mdpi.com/2079-4983/14/7/368Test; https://doaj.org/toc/2079-4983Test |
DOI: | 10.3390/jfb14070368 |
الوصول الحر: | https://doaj.org/article/a7877c18435c4ccebed341965bb14e03Test |
رقم الانضمام: | edsdoj.7877c18435c4ccebed341965bb14e03 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 20794983 |
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DOI: | 10.3390/jfb14070368 |