دورية أكاديمية
Towards a Precise NMR Quantification of Acute Phase Inflammation Proteins from Human Serum
| العنوان: | Towards a Precise NMR Quantification of Acute Phase Inflammation Proteins from Human Serum |
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| المؤلفون: | Mallagaray, Álvaro, Rudolph, Lorena, Lindloge, Melissa, Mölbitz, Jarne, Thomsen, Henrik, Schmelter, Franziska, Alhabash, Mohamad Ward, Abdullah, Mohammed R., Saraei, Roza, Ehlers, Marc, Graf, Tobias, Sina, Christian, Petersmann, Astrid, Nauck, Matthias A., Günther, Ulrich L. |
| سنة النشر: | 2023 |
| المجموعة: | Publikationsdatenbank der Fraunhofer-Gesellschaft |
| مصطلحات موضوعية: | Acute Phase Proteins, Diagnostic Tool, GlycA/B, Glycoproteins, NMR, NMR Blood Analyses, NMR Metabolomics |
| الوصف: | Nuclear Magnetic Resonance (NMR) spectra of human serum and plasma show, besides metabolites and lipoproteins, two characteristic signals termed GlycA and B arising from the acetyl groups of glycoprotein glycans from acute phase proteins, which constitute good markers for inflammatory processes. Here, we report a comprehensive assignment of glycoprotein glycan NMR signals observed in human serum, showing that GlycA and GlycB signals originate from Neu5Ac and GlcNAc moieties from N-glycans, respectively. Diffusion-edited NMR experiments demonstrate that signal components can be associated with specific acute phase proteins. Conventionally determined concentrations of acute phase glycoproteins correlate well with distinct features in NMR spectra (R2 up to 0.9422, p-value <0.001), allowing the simultaneous quantification of several acute phase inflammation proteins. Overall, a proteo-metabolomics NMR signature of significant diagnostic potential is obtained within 10–20 min acquisition time. This is exemplified in serum samples from COVID-19 and cardiogenic shock patients showing significant changes in several acute phase proteins compared to healthy controls. ; 62 ; 35 |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 14337851 |
| العلاقة: | Angewandte Chemie. International edition; https://publica.fraunhofer.de/handle/publica/450976Test |
| DOI: | 10.1002/anie.202306154 |
| الإتاحة: | https://doi.org/10.1002/anie.202306154Test https://publica.fraunhofer.de/handle/publica/450976Test |
| حقوق: | open access |
| رقم الانضمام: | edsbas.9C3E956F |
| قاعدة البيانات: | BASE |
| تدمد: | 14337851 |
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| DOI: | 10.1002/anie.202306154 |