Comparison of HbA1c detection in whole blood and dried blood spots using an automated ion-exchange HPLC system
| العنوان: | Comparison of HbA1c detection in whole blood and dried blood spots using an automated ion-exchange HPLC system |
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| المؤلفون: | Elise Casteleyn, Orianne Villard, Jacques Ducos, Sylvain Lehmann, Antoine Avignon, Christophe Hirtz, Ariane Sultan, Aleksandra Maceski Maleska |
| المساهمون: | Cellules Souches, Plasticité Cellulaire, Médecine Régénératrice et Immunothérapies (IRMB), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Pathogénèse et contrôle des infections chroniques (PCCI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier ), Laboratoire de Virologie [CHRU Montpellier], Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Herrada, Anthony, Centre Hospitalier Universitaire de Montpellier (CHU Montpellier )-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), Laboratoire de Virologie - CHRU Montpellier, Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | Bioanalysis Bioanalysis, 2017, 9 (5), pp.427-434. ⟨10.4155/bio-2016-0278⟩ Bioanalysis, Future Science, 2017, 9 (5), pp.427-434. ⟨10.4155/bio-2016-0278⟩ |
| بيانات النشر: | HAL CCSD, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Male, Clinical Biochemistry, DBS, ion exchange, 030204 cardiovascular system & hematology, Analytical Chemistry, Automation, 0302 clinical medicine, General Pharmacology, Toxicology and Pharmaceutics, Dried blood, [SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Chromatography, High Pressure Liquid, Whole blood, clinical biology, Blood Specimen Collection, Venipuncture, Ion exchange hplc, Protein Stability, General Medicine, Middle Aged, Chromatography, Ion Exchange, dried blood spot, 3. Good health, Dried blood spot, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], Medical Laboratory Technology, surgical procedures, operative, ISO15189, Female, Adult, Analyte, HbA1c, Complete Method, 030209 endocrinology & metabolism, 03 medical and health sciences, Diabetes mellitus, medicine, Humans, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], Aged, Chromatography, business.industry, method validation, medicine.disease, Diabetes Mellitus, Type 1, Diabetes Mellitus, Type 2, Case-Control Studies, Dried Blood Spot Testing, HPLC, business, Biomarkers, glycated hemoglobin |
| الوصف: | International audience; AIM: Hemoglobin A1c (HbA1c) is a widely recognized analyte for diagnosing and monitoring diabetes. Dried blood spot (DBS) constitutes a useful alternative to blood collection by venipuncture. Analytical and clinical validation of DBS use is, however, necessary before implementation.Results/methodology: HbA1c levels from whole blood or DBS from a cohort patients with diabetes were compared. DBS specimens were stable at ambient temperature. HbA1c detection on DBS was accurate, robust, and the correlation and agreement with whole blood values was excellent.CONCLUSION: this study provides for the first time a complete method comparison and validation under the ISO15189 guideline using an automated HPLC system. This approach constitutes, therefore, a useful tool for diagnosing diabetes. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1757-6180 1757-6199 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ecf74d0550e01ef3e5d0747fdca165bTest https://hal.science/hal-01781609/documentTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3ecf74d0550e01ef3e5d0747fdca165b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17576180 17576199 |
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