Phytomodulatory proteins isolated from Calotropis procera latex promote glycemic control by improving hepatic mitochondrial function in HepG2 cells
| العنوان: | Phytomodulatory proteins isolated from Calotropis procera latex promote glycemic control by improving hepatic mitochondrial function in HepG2 cells |
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| المؤلفون: | Hygor N. Araujo, Ariclécio Cunha de Oliveira, Regina de Jesus das Neves, Tanes I. Lima, Renata Prado Vasconcelos, Paula Alexandre de Freitas, Keciany Alves de Oliveira, Marina Gabrielle Guimarães de Almeida, André Gustavo de Oliveira, Márcio V. Ramos, Leonardo R. Silveira, Bianca Cristine Favero-Santos, Dimitrius Santiago P.S.F. Guimarães |
| المصدر: | Saudi Pharmaceutical Journal : SPJ Saudi Pharmaceutical Journal, Vol 29, Iss 9, Pp 1061-1069 (2021) |
| بيانات النشر: | Elsevier, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Proteases, Latex, HGP, Hepatic glucose production, Pharmaceutical Science, RM1-950, Pharmacology, PPAR, Peroxisome proliferator-activated receptor, UCP2, Mitochondrial uncoupling protein 2, MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, FCCP, Oligomycin carbonyl cyanide 4 (trifluoromethoxy) phenylhydrazine, Calotropis procera, Bioactive proteins, Gene expression, Viability assay, PPRE, PPAR response element, Protein kinase A, Glycemic, chemistry.chemical_classification, Reactive oxygen species, CpPII, Major peptidase fraction treated with iodoacetamide, biology, Chemistry, AMPK, AMP-activated kinase protein, AMPK, DMEM, Dulbecco’s minimal essential medium, DMSO, Dimethyl sulfoxide, DHE, Dihydroethidium, OXPHOS, Oxidative phosphorylation, TBS-T, Tris buffered saline solution containing 0.1% Tween 20, biology.organism_classification, AUC, Area under the curve, Folk medicine, OCR, Oxygen consumption rate, CTL, Control, Original Article, Therapeutics. Pharmacology, Glycemia, LP, Soluble latex proteins from Calotropis procera, ROS, Reactive oxygen species |
| الوصف: | The medicinal uses of Calotropis procera are diverse, yet some of them are based on effects that still lack scientific support. Control of diabetes is one of them. Recently, latex proteins from C. procera latex (LP) have been shown to promote in vivo glycemic control by the inhibition of hepatic glucose production via AMP-activated protein kinase (AMPK). Glycemic control has been attributed to an isolated fraction of LP (CpPII), which is composed of cysteine peptidases (95%) and osmotin (5%) isoforms. Those proteins are extensively characterized in terms of chemistry, biochemistry and structural aspects. Furthermore, we evaluated some aspects of the mitochondrial function and cellular mechanisms involved in CpPII activity. The effect of CpPII on glycemic control was evaluated in fasting mice by glycemic curve and glucose and pyruvate tolerance tests. HepG2 cells was treated with CpPII, and cell viability, oxygen consumption, PPAR activity, production of lactate and reactive oxygen species, mitochondrial density and protein and gene expression were analyzed. CpPII reduced fasting glycemia, improved glucose tolerance and inhibited hepatic glucose production in control animals. Additionally, CpPII increased the consumption of ATP-linked oxygen and mitochondrial uncoupling, reduced lactate concentration, increased protein expression of mitochondrial complexes I, III and V, and activity of peroxisome-proliferator-responsive elements (PPRE), reduced the presence of reactive oxygen species (ROS) and increased mitochondrial density in HepG2 cells by activation of AMPK/PPAR. Our findings strongly support the medicinal use of the plant and suggest that CpPII is a potential therapy for prevention and/or treatment of type-2 diabetes. A common epitope sequence shared among the proteases and osmotin is possibly the responsible for the beneficial effects of CpPII. |
| اللغة: | English |
| تدمد: | 2213-7475 1319-0164 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::589b5f0bac9dfc1fd17b68bf7d99c68aTest http://europepmc.org/articles/PMC8463474Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....589b5f0bac9dfc1fd17b68bf7d99c68a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22137475 13190164 |
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