التفاصيل البيبلوغرافية
| العنوان: |
同型半胱氨酸致胰岛β 细胞凋亡中肝配蛋白A 型受体2 DNA 甲基化升高. (Chinese) |
| العنوان البديل: |
Ephrin A receptor 2 DNA methylation increases in pancreatic beta cell apoptosis induced by homocysteine. (English) |
| المؤلفون: |
张 晴, 高春兰, 于飞飞, 张正皓, 马 芳, 高 源, 李桂忠, 姜怡邓, 马胜超 |
| المصدر: |
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu; 2/18/2023, Vol. 27 Issue 5, p714-719, 6p |
| مصطلحات موضوعية: |
EPHRIN receptors, DNA methylation, BAX protein, PROTEIN expression, ISLANDS of Langerhans |
| الملخص (بالإنجليزية): |
BACKGROUND: Increased homocysteine levels lead to apoptosis of pancreatic β cells, but the exact mechanism remains unclear. OBJECTIVE: To explore the specific mechanism of DNA hypermethylation of Ephrin A receptor 2 (EphA2) and its promoter region in pancreatic β cells. METHODS: Mouse insulinoma cell lines (Min6) were cultured in vitro and divided into control group (0 µmol/L homocysteine) and homocysteine group (120 µmol/L homocysteine). After 48 hours of intervention in the cells, immunofluorescence and western blot were used to test the expression of apoptosisrelated proteins Bax, Bcl-2, and Caspase-3 in pancreatic islet β cells of the two groups. The expression levels of DNA methylation-related proteins DNMT1 and DNMT3a were detected by western blot. Real-time fluorescent quantitative PCR (qRT- PCR) was used to detect the level of EphA2 mRNA. Western blot was used to detect the protein expression of EphA2. Nested methylation-specific PCR was used to detect the level of DNA methylation in the promoter region of EphA2. RESULTS AND CONCLUSION: Compared with the control group, the expression of apoptosis-related proteins Bax and Caspase-3 in the pancreatic β cells was significantly increased in the homocysteine group, and the expression of Bcl-2 was significantly decreased; the mRNA and protein expression levels of EphA2 were significantly decreased (P < 0.05). Compared with the control group, the EphA2 DNA methylation level and the expression of DNMT1 protein in the pancreatic β cells were significantly higher in the homocysteine group (P < 0.05). To conclude, EphA2 DNA hypermethylation plays a significant role in homocysteine-induced pancreatic β cell apoptosis and DNMT1 may be involved in its hypermethylation process [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
背景：同型半胱氨酸水平增加会导致胰岛β细胞发生凋亡，但其具体机制尚不明确。 目的：探讨胰岛β细胞中肝配蛋白A型受体2及其启动子区DNA高甲基化的具体机制。 方法：体外培养小鼠胰岛β细胞株Min6，将其分为对照组(0 μmol/L同型半胱氨酸)和同型半胱氨酸组(120 μmol/L同型半胱氨酸)。干预细胞 48 h后，采用免疫荧光和Western blot法检测2组细胞中凋亡相关蛋白Bax、Bcl-2、半胱氨酰天冬氨酸特异性蛋白酶3表达情况；Western blot 法检测DNA甲基化相关蛋白DNMT1、DNMT3a的表达水平；实时荧光定量PCR检测两组细胞中肝配蛋白A型受体2 mRNA水平；Western blot 检测肝配蛋白A型受体2的蛋白表达情况；巢式甲基化特异性PCR检测EphA2启动子区DNA甲基化水平。 结果与结论：①与对照组相比，同型半胱氨酸组胰岛β细胞中凋亡相关蛋白Bax和半胱氨酰天冬氨酸特异性蛋白酶3表达明显升高，Bcl-2表 达明显下降；肝配蛋白A型受体2的mRNA和蛋白表达水平明显下降(P < 0.05)； ②与对照组相比，同型半胱氨酸组肝配蛋白A型受体2 DNA甲 基化水平明显升高(P < 0.05)，同型半胱氨酸组胰岛β细胞中DNMT1蛋白表达明显增高(P < 0.05)；③提示肝配蛋白A型受体2 DNA高甲基化在 同型半胱氨酸致胰岛β细胞凋亡中的作用明显，而DNMT1可能参与其高甲基化过程。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
