التفاصيل البيبلوغرافية
| العنوان: |
锶和氮改性二氧化钛/纳米羟基磷灰石复合树脂的制备和性能. (Chinese) |
| العنوان البديل: |
Preparation and properties of strontium and nitrogen co-doped titanium dioxide/nano hydroxyapatite composites resins. (English) |
| المؤلفون: |
赵远航, 闫琳琳, 宋嘉卓, 邹馨颖, 赵 红, 刘 新, 陈佳文, 于依岩, 张志民, 张 红 |
| المصدر: |
Journal of Jilin University (Medicine Edition); Jul2023, Vol. 49 Issue 4, p1067-1075, 9p |
| مصطلحات موضوعية: |
BACTERIA morphology, ARTIFICIAL saliva, SCANNING electron microscopes, INFRARED absorption, DOUBLE bonds |
| الملخص (بالإنجليزية): |
Objective: To prepare the strontium and nitrogen co-doped titanium dioxide (Sr-N-TiO2)/ nano hydroxyapatite (n-HA) composite resins, to evaluate its basic properties, antibacterial properties, remineralization abilities, and biosafeties, and to clarify the antibacterial mechanism of the new type of composite resins. Methods: Sr-N-TiO2 and n-HA were mixed as the reinforcing fillers. The composite resins were prepared and were divided into control group and three experimental groups. According to the mass fractions of reinforcing fillers, the experimental groups were divided into 2. 5% reinforcing filler group, 5. 0% reinforcing filler group, and 7. 5% reinforcing filler group. The infrared spectrogram of composite resins in various groups were detected by the Fourier-transform infrared absorption spectrogram and the double bond conversion rates were calculated before and after light curing for 60 s. The curing depths of composite resin specimens in various groups after light curing for 20 s were calculated by cylindrical mould (height=10. 0 mm, diameter=4. 0 mm). Three composite resin specimens in various groups were prepared and co-cultured with the dilute Streptococcus mutans solution. The plate colony counting method was used to determine the counts of attached bacteria on surface of the specimens and the antibacterial rates were calculated; live/dead bacteria staining method was used to observe the ratio of live bacteria/dead bacteria and morphology of composite resin speciments in various groups. The composite resin specimens were immersed in the artificial saliva for 1, 3, 5, and 7 d, and the mineralizations of surface of the composite resins were observed under scanning electron microscope (SEM). The mouse fibroblast cells (L-929 cells) were incubated with the resin extract solution, and CCK-8 method was used to detect the relative growth rates (RGR) of the cells on the 1st, 2nd, and 3rd days and the cytotoxicity levels were evaluated. Results: With the increasing of reinforcing filler proportion of the composite resins, the double bond conversion rates and curing depths of new type of the composite resins showed a decreasing trend, but all reached the clinical standard. Compared with control group, the double bond conversion rates of new type of the composite resins in 2. 5% reinforcing filler group and 5. 0% reinforcing filler group had no significant differences (P>0. 05), and the above index in 7. 5% reinforcing filler group was decreased (P< 0. 05). Compared with control group, the curing depths of the composite resins in experimental groups were increased (P<0. 05 or P<0. 01), the counts of attached bacteria on surface of the speciments in experimental groups were significant increased (P<0. 01). When the proportion of reinforcing fillers reached 5. 0%, the antibacterical rates of new type of the composite resins were higher than 90%. Compared with control group, the counts of live bacteria on surface of the composite resins in experimental groups were decreased with the increasing of proportion of the reinforcing fillers. The SEM results show that the mineralized nodules on surface of the composite resins in experimental groups could be observed, and the number of mineralized nodules was increased with the prolongation of time and the increasing of reinforcing fillers proportion. After treated for 7 d, the surface of the composite resins in 5. 0% reinforcing filler group and 7. 5% reinforcing filler group were almostly covered by mineralized nodules. The biosafety experiment results showed that the RGR of cells in various groups was above 75%, and the cytotoxicity level ≤1 grade. Conclusion: The modified new type of composite resins meet the clinical standards, and it has antibacterial and remineralization properties, as well as high biosafety. [ABSTRACT FROM AUTHOR] |
| Abstract (Chinese): |
目的：制备锶和氮改性二氧化钛 (Sr-N-TiO2) /纳米羟基磷灰石 (n-HA) 复合树脂， 并对其基础性能、抗菌性能、再矿化能力和生物安全性进行评价，阐 明 新 型 复 合 树 脂 的 抗 菌 机 制。 方法：Sr-N-TiO2 与 n-HA 组合作为增强填料，制备复合树脂，分为不含增强填料的对照组和 3 个实验 组，根据实验组新型复合树脂中增强填料质量分数不同又分为 2. 5% 增强填料组、5. 0% 增强填料组 和 7. 5% 增强填料组。采用傅里叶红外吸收光谱分别检测光固化 60 s 前后各组复合树脂的红外光谱， 计算复合树脂双键转化率。使用直径 4 mm、高 10 mm 的模具制备各组复合树脂试件，计算光照 20 s 后各组复合树脂试件固化深度。每组制备 3 个复合树脂试件，将各组试件与稀释的变异链球菌液共培 养，通过平板菌落计数法检测各组试件表面黏附细菌菌落数 并 计 算 抗 菌 率 ， 采 用 活 /死 细 菌 染 色法 观察各组复合树脂试件表面活/死细菌比例及形态表现。将各组复合树脂试件于人工唾液中浸泡 1、3、 5 和 7 d，采用扫描电子显微镜 (SEM) 观察各组试件表面再矿化情况。小鼠纤维细胞 (L-929 细胞) 接种于各组复合树脂浸提液中，采用 CCK-8 法分别于 1、2 和 3 d 时检测各组细胞相对增殖率 (RGR)， 进行细胞毒性等级评价。结果：随着复合树脂中增强填料比例的增加，新型复合树脂双键转化率和固 化深度呈降低趋势，但均达到临床使用标准。与对照组比较，2. 5% 和 5. 0% 增强填料组新型复合树 脂双键转化率差异无统计学意义 (P>0. 05)，7. 5% 增强填料组新型复合树脂双键转化率明显降低 (P<0. 05)。与对照组比较，各实验组新型复合树脂固化深度明显降低 (P<0. 05 或 P<0. 01)，表面 黏附细菌菌落计数明显减少 (P<0. 01)。当增强填料比例达到 5. 0% 时，新型复合树脂抗菌率大于 90%。与对照组比较，各实验组新型复合树脂试件表面活细菌数随增强填料比例升高逐渐减少。SEM 观察，各实验组新型复合树脂试件表面可见矿化结节，并随时间延长和增强填料比例升高而增多，处 理 7 d 后，5. 0% 和 7. 5% 增强填料组新型复合树脂试件表面几乎被矿化结节完全覆盖。生物安全性实 验，各组细胞 RGR 均大于 75%，细胞毒性等级均≤1 级。结论：改性后的新型复合树脂符合临床标 准，并具有抗菌性能和再矿化能力，同时生物安全性较高。 [ABSTRACT FROM AUTHOR] |
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| قاعدة البيانات: |
Complementary Index |
